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羊奶果不同发育阶段根瘤的细胞结构及固氮、吸氢活性
引用本文:刘育梅,黄维南,黄志宏,刘剑秋.羊奶果不同发育阶段根瘤的细胞结构及固氮、吸氢活性[J].热带亚热带植物学报,2003,11(1):23-26.
作者姓名:刘育梅  黄维南  黄志宏  刘剑秋
作者单位:1. 福建省亚热带植物研究所,福建,厦门,361006;福建师范大学生物工程学院,福建,福州,350007
2. 福建省亚热带植物研究所,福建,厦门,361006
3. 福建师范大学生物工程学院,福建,福州,350007
摘    要:比较羊奶果根瘤三个不同发育阶段的显微,亚显微结构和固氮,吸氢活性的差异。探讨了根瘤结构与功能的关系。结果表明:早期侵染方式为皮层细胞间隙侵染,此期的内生菌是一种分枝,具隔膜的菌丝体,早期侵染细胞有脂体存在。成熟根瘤含菌细胞明显多于幼瘤和衰老瘤。成熟根瘤具有大量泡囊,成熟泡囊具分隔,双层壁结构。衰老瘤泡囊分隔消失,不呈双层壁结构。成熟根瘤的固氮,吸氢活性明显高于幼瘤和衰老瘤。

关 键 词:羊奶果  根瘤  亚显微结构  固氮活性  吸氢活性  放线菌
文章编号:1005-3395(2003)01-0023-04

Cellular Structure of Root Nodules of Elaeagnus conferta Roxb. and Nitrogen Fixation Activity
LIU Yu-mei,HUANG Wei-nan,HUANG Zhi-hong,LIU Jian-qiu.Cellular Structure of Root Nodules of Elaeagnus conferta Roxb. and Nitrogen Fixation Activity[J].Journal of Tropical and Subtropical Botany,2003,11(1):23-26.
Authors:LIU Yu-mei  HUANG Wei-nan  HUANG Zhi-hong  LIU Jian-qiu
Abstract:The ultrastructure of root nodules of Elaeagnus confe r ta Roxb. and the activities of nitrogen fixation and hydrogen uptake were s tud ied. The results show that Frankia hyphae exist at early stage in int erce llular space in nodule cortex. The filamentous hyphae are branched with se pta. Lipid bodies are distributed in infected young cells. The number of infect ed cel ls in mature nodules is much larger than that of young and senescent nodu les.Apical endophytic hyphae swell to form lots of vesicles which are septate and do uble-walled. Vesicles are suggested to be importatant in nitrogen fixation. In s enescent nodules, the vesicles disintegrate. Nitrogen fixation and hydrogen up ta ke activities in mature nodule is much higher than that in young and senescen t n odules.
Keywords:Elaeagnus conferta  Root nodule  Ultrastructure  Nit  rogen fixation activity  Hydrogen uptake activity
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