Genetic diversity and structure of natural Pinus tabulaeformis populations in North China using amplified fragment length polymorphism (AFLP) |
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| Institution: | 1. Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen DK-2200, Denmark;2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M DK-5230, Denmark;3. Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark;4. Department of Nutrition, University of California Davis, Davis, CA 95616, USA;5. Department of Internal Medicine, University of California Davis, Sacramento, CA 95817, USA;6. National Institute of Nutrition and Seafood Research (NIFES), Bergen N-5817, Norway;1. Instituto Universitario de Electroquímica, Universidad de Alicante, Apdo. 99, Alicante E-03080, Spain;2. Área de electroquímica y corrosión, INTEMA (CONICET), Juan B. Justo 4302, B7608FDQ Mar del Plata, Argentina |
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| Abstract: | Chinese pine (Pinus tabulaeformis carr.), endemic to China, is a conifer species with extensive and fragmented distribution in North China. In this study, the genetic diversity and structure of 20 natural populations of this species were investigated using amplified fragment length polymorphism (AFLP) markers. A total of 445 fragments were revealed with 8 pairs of primers, 379 (85.17%) of which were polymorphic. A moderate level of genetic diversity was detected at the species level (Shannon's information index I = 0.356, Nei's gene diversity HE = 0.271) and at the population level (I = 0.219, HE = 0.206). Most of genetic variation was within populations while a considerable level of genetic differentiation was detected (GST = 0.352, ФST = 0.304). The high differentiation could be attributed to the complex and fragmented habitats, and a limited gene flow among populations (Nm = 0.572). The Mantel test indicated that there was significant correlation (r = 0.455, P < 0.001) between Nei's genetic distance and geographical distance among all the populations. The results suggested that proper countermeasures should be taken to prevent the habitat further deterioration and maintain the genetic diversity of this species. |
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| Keywords: | AFLP Genetic diversity Genetic differentiation |
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