Characteristics of arylsulfatase in Russian sturgeon (Acipenser gueldenstaedti) semen |
| |
| Authors: | Sarosiek Beata Ciereszko Andrzej Kolman Ryszard Glogowski Jan |
| |
| Institution: | a Department of Molecular Andrology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, ul. Bydgoska 1/8, 10-243, Olsztyn, Poland;b Semen Biology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, ul. Bydgoska 1/8, 10-243, Olsztyn, Poland;c Inland Fisheries Institute, Olsztyn, Poland |
| |
| Abstract: | Spermatozoa of sturgeons (Acipenseriformes), unlike teleosts, possess an acrosome. This paper provides data concerning biochemical characteristics of arylsulfatase (AS), an acrosomal enzyme, found in Russian sturgeon spermatozoa and seminal plasma. The enzymes were purified by a four-step procedure, using n-butanol extraction, ion-exchange chromatography repeated twice and gel filtration. High purity of our enzymes was confirmed by silver staining electrophoresis and an immunological experiment. Kinetic parameters indicated that the purified enzymes belong to arylsulfatase type A. Similarity of the seminal plasma arylsulfatase to the spermatozoan enzyme showed us that arylsulfatase from seminal plasma might originate from damaged spermatozoa. The possible physiological consequences of the presence of arylsulfatase in Russian sturgeon semen are discussed. |
| |
| Keywords: | Arylsulfatase Milt plasma Russian sturgeon Spermatozoa Acrosome Semen Acipenseridae Milt |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
