| 染色体显微切割与DOP—PCR结合对赤麂Sry基因克隆，测序及初步定位 |
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| 引用本文： | 张悦,鲁晓萱,刘宁生,单祥年,余多慰.染色体显微切割与DOP—PCR结合对赤麂Sry基因克隆，测序及初步定位[J].遗传学报,2001,28(4):322-326. |
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| 作者姓名： | 张悦 鲁晓萱 刘宁生 单祥年 余多慰 |
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| 作者单位： | 1. 东南大学医学院生物教研室,
2. 南京师范大学生命科学院, |
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| 基金项目： | 国家自然科学基金资助(No.39670393) |
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| 摘 要： | 应用显微切割技术获得赤麂1号，Y1，Y2染色体，通过DOP－PCR增加模板DNA拷贝数，然后用人的性别决定基因（Sex-tetermininig Region of the Chromosome Y,SRY)中HMG框内设计1对引物，对DOP－PCR产物进行扩增，在雄性赤麂Y2染色体DOP－PCR产物中扩增出与人SRY基因同源的Sry基因片段，克隆，测序，首次在分子水平上证明赤麂Y2染色体是真正的Y染色体，同时对赤麂Syr基因进行了初步定位。
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| 关 键 词： | 染色体 显微切割 DOP－PCR Sry基因 赤麂 性染色体 基因定位 |
| 文章编号： | 0379-4172(2001)04-0322-05 |
| 修稿时间： | 2000年7月10日 |
Cloning, Sequencing and
Chromosome Location of Sry Gene of Muntjak munticus vaginalis by DOP-PCR and
Microdissection |
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| ZHANG Yue,LU Xiao-xuan,LIU Ning-sheng,SHAN Xiang-nian,YU Duo-wei.Cloning, Sequencing and
Chromosome Location of Sry Gene of Muntjak munticus vaginalis by DOP-PCR and
Microdissection[J].Journal of Genetics and Genomics,2001,28(4):322-326. |
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| Authors: | ZHANG Yue LU Xiao-xuan LIU Ning-sheng SHAN Xiang-nian YU Duo-wei |
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| Abstract: | We isolated 1, Y1, Y2 chromosomes of the male M.m vaginalis by microdissection and amplified the DNA copies by DOP-PCR. Using the DOP-PCR products of 1, Y1, Y2 chromosomes of M.m vaginalis as template respectively and the primers designed within human SRY HMG box, the Sry gene of the male M.m vaginalis was amplified, and it was cloned and sequenced. It is proved that Y2 is the real sex chromosome in the male M.m vaginalis at molecular level for the first time, and Sry was localized on Y2 chromosome. |
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| Keywords: | microdissection DOP-PCR Sry gene M m vaginalis sex chromosome chromosome location |
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