芒果SEPALAATA基因的克隆与表达分析

以芒果品种‘吕宋’(Mangifera indica L.Carabao)为试材,利用同源克隆和RACE技术从花序中获得了1个芒果SEPALAATA(SEP)基因cDNA全长,命名为MSEP1(GenBank中登录号为KP702299)。MSEP1基因的cDNA全长为921bp,包含一个长度为726bp开放阅读框,编码241个氨基酸,蛋白质相对分子质量为27.7kD,理论等电点为5.79。序列比对和系统进化树分析表明,MSEP1具有保守的MADS-box及半保守的K区,属于MADS-box家族的SEP亚家族。器官特异性表达分析表明,MSEP1基因在芒果根、茎中表达量较低,在叶片、花芽中表达量较高,而在花序中表达量最高。研究推测,MSEP1基因可能在芒果生殖生长中发挥重要作用。

Cloning and Expression Analysis of SEPALAATA Gene in Mango (Mangifera indica L.)

A full length cDNA sequence of SEPALAATA(SEP) gene was obtained from inflorescence tissue of mango (Mangifera indica cv. Carabao) with homology based cloning and RACE, named MSEP1 (GenBank accession no.KP702299). The full length of MSEP1 is 921 bp containing 726 bps open reading frame, encoding 241 amino acids with molecular weight 27.7 kD and isoelectric point 5.79. Sequence alignment and phylogenetic tree analysis indicated that MSEP1 deduced protein contained a conservative MADS box and semi conservative K domain and belonged to the SEP subfamily of the MADS box family. Tissue specific analysis showed that MSEP1 was high expression in leaves and flowers especially in inflorescences, low expression in roots and stems of mango. MSEP1 may have important roles in reproductive growth of mango.