饥饿与重摄食对河蟹肠道菌群结构的影响

研究采用16S rRNA测序研究饥饿以及重摄食的河蟹(Eriocheir sinensis)肠道菌群结构变化, 结果显示, 在饥饿胁迫下, 河蟹肠道细菌群落Alpha多样性指数下降, 重摄食后仍未改变肠道菌群多样性指数下降的趋势, 但与对照组无显著差异(P>0.05); 而在菌门组成方面, 随着饥饿时间的增加, 4个优势菌门占比呈现了相应的变化, 变形菌门、厚壁菌门以及拟杆菌门占比逐渐增加, 软壁菌门占比逐渐下降, 但在重摄食后, 4个优势菌门恢复至对照组水平; 而通过对比不同状态下差异菌群, 在科属水平下筛选出8个具有显著差异(P< 0.05)的菌群, 其中Candidatus Bacilloplasma菌属因具有较高的菌群丰度, 且对饥饿以及重摄食响应较为显著, 其菌群的特定功能值得进一步深究。研究首次报道了饥饿状态以及重摄食后河蟹肠道菌群的变化, 对于后续探究菌群的特定功能提供基础数据。

EFFECTS OF STARVATION AND REFEEDING ON INTESTINAL MICROFLORA OF CHINESE MITTEN CRAB (ERIOCHEIR SINENSIS)

The interplay between intestinal microorganism and their host has received extensive scientific attention, however, there is no study about the response of intestinal microorganism of the Chinese mitten crab in the hunger state. In the present study, 16S rRNA sequencing was used to investigate intestinal microorganism in Chinese mitten crab during the period of starvation and refeeding. The results showed that the Alpha diversity index of the intestinal microorganisms decreased by starvation, and re-feeding did not change the descending trend of the intestinal microbial diversity index. Besides, there was no significant difference comparing to the control group (P>0.05). In terms of the bacterial phyla composition, starvation gradually increased the proportions of the Proteobacteria, Firmicutes and Bacteroidetes and reduced the proportion of Tenericutes. Re-feeding restored Proteobacteria, Firmicutes, Bacteroidetes and Tenericutes to the level of control group. By comparing different strains under different conditions, 8 strains with significant differences (P<0.05) were sifted at the family and genus level. Among them, Candidatus bacilloplasma was characterized by high abundance of bacteria and significant response to starvation and refeeding, and its specific function of the flora needs further investigation. This study first reported the changes of intestinal flora of the crabs after starvation and refeeding, which could provide basic knowledge for the further exploration of the specific functions of the flora.