Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut |
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Authors: | Geetha Govind Harshavardhan Vokkaliga ThammeGowda Patricia Jayaker Kalaiarasi Dhanalakshmi Ramchandra Iyer Senthil Kumar Muthappa Sreenivasulu Nese Udaya Kumar Makarla |
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Institution: | 1.Department of Crop Physiology,University of Agricultural Sciences, GKVK,Bangalore,India;2.Monsanto Research Centre,Bangalore,India;3.Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK),Gatersleben,Germany;4.Max Planck Institute for Chemical Ecology,Jena,Germany |
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Abstract: | Peanut, found to be relatively drought tolerant crop, has been the choice of study to characterize the genes expressed under
gradual water deficit stress. Nearly 700 genes were identified to be enriched in subtractive cDNA library from gradual process
of drought stress adaptation. Further, expression of the drought inducible genes related to various signaling components and
gene sets involved in protecting cellular function has been described based on dot blot experiments. Fifty genes (25 regulators
and 25 functional related genes) selected based on dot blot experiments were tested for their stress responsiveness using
northern blot analysis and confirmed their nature of differential regulation under different field capacity of drought stress
treatments. ESTs generated from this subtracted cDNA library offered a rich source of stress-related genes including signaling
components. Additional 50% uncharacterized sequences are noteworthy. Insights gained from this study would provide the foundation
for further studies to understand the question of how peanut plants are able to adapt to naturally occurring harsh drought
conditions. At present functional validation cannot be deemed in peanut, hence as a proof of concept seven orthologues of
drought induced genes of peanut have been silenced in heterologous N. benthamiana system, using virus induced gene silencing method. These results point out the functional importance for HSP70 gene and key
regulators such as Jumonji in drought stress response.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
The nucleotide sequence reported in this paper has been submitted to (database) under accession numbers: Acc. No. EC268400–EC268685;
EC365167–EC365455.
An erratum to this article can be found at |
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Keywords: | Peanut Drought tolerance Functional genomics EST VIGS |
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