| 荔枝果皮总蛋白质提取及双向电泳体系的建立 |
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| 引用本文： | 李开拓,郭志雄,潘东明,钟凤林,潘腾飞.荔枝果皮总蛋白质提取及双向电泳体系的建立[J].热带亚热带植物学报,2011,19(1):69-74. |
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| 作者姓名： | 李开拓 郭志雄 潘东明 钟凤林 潘腾飞 |
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| 作者单位： | 福建农林大学园艺学院,福州,350002 |
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| 基金项目： | 国家科技支撑计划项目(2007BAD07B01) |
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| 摘 要： | 用TCA-丙酮、丙酮和酚3种方法提取荔枝(Litchi chinensis Sonn.)果皮的总蛋白质,比较了蛋白产量、单向SDS-PAGE和双向电泳等方面的差异,并对双向电泳体系进行探索.结果表明:酚抽提法最佳,提取的总蛋白得率最高,蛋白在单向SDS-PAGE中形成条带数目最多,最清晰;经双向电泳分离用银染显色,可检...
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| 关 键 词： | 荔枝 果皮 蛋白质组 提取 双向电泳 |
| 收稿时间： | 2010/3/30 0:00:00 |
| 修稿时间： | 2010/8/22 0:00:00 |
Extraction of Total Protein from Litchi Pericarps and Establishment of Two-dimensional Electrophoresis |
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| LI Kai-tuo,GUO Zhi-xiong,PAN Dong-ming,ZHONG Feng-lin and PAN Teng-fei.Extraction of Total Protein from Litchi Pericarps and Establishment of Two-dimensional Electrophoresis[J].Journal of Tropical and Subtropical Botany,2011,19(1):69-74. |
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| Authors: | LI Kai-tuo GUO Zhi-xiong PAN Dong-ming ZHONG Feng-lin and PAN Teng-fei |
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| Institution: | College of Horticulture, Fujian Agriculture and Forestry University,College of Horticulture, Fujian Agriculture and Forestry University,College of Horticulture, Fujian Agriculture and Forestry University,College of Horticulture, Fujian Agriculture and Forestry University,College of Horticulture, Fujian Agriculture and Forestry University |
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| Abstract: | The total protein was extracted from Lichi chinensis pericarp by using three methods, such as trichloroacetic acid (TCA)-acetone, acetone and phenol. The results showed that the total protein yield was the highest by phenol extraction method, and protein bands were the most and clear in one dimentional SDS-PAGE. About 870 clear protein spots were detected by two-dimensional electrophoresis (2-DE) and silver staining with isoelectric points at pH 4~8, and relative molecular weight of protein ranged from 15.0 to 85.0 kD. |
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| Keywords: | Litchi chinensis Sonn Pericarp Proteome Extraction 2-DE |
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