蛋白质突变体基因库构建方法的研究进展

体外定向进化是蛋白质工程中一个非常有效的设计策略。最近几年，在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。本文对这些方法及其特点加以总结，为解决特定问题选取何种方法提供一定依据。最近研究表明：定向进化和理性设计相结合、定向进化和以结构为基础的计算设计方法相结合正成为蛋白质工程中两个新的发展方向。

Progress in Construction Methods of Diverse Mutated Gene Libraries for Protein

Directed evolution has been proven to be an effective method for proteins engineering. In the past few years, many new protocols for directed evolution were developed on the basis of oligonucleotide -directed random mutation, error-prone PCR and DNA shuffling. These new methods and their characteristics were summarized to provide a reference for choosing an approach to modify specific protein properties. The recent researches indicated that the combined directed evolution, coupling the directed evolution with rational design and computational design approaches respectively, is becoming increasingly important in protein engineering and is becoming the new direction for directed evolution.