| 固定对组织光学性质的影响 |
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| 引用本文： | 张镇西,扬枫.固定对组织光学性质的影响[J].激光生物学报,1993,2(1):204-206. |
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| 作者姓名： | 张镇西 扬枫 |
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| 作者单位： | 中国科技大学物理系，中国科技大学物理系，中国科技大学物理系，安徽医科大学药理教研室，安徽医科大学药理教研室，安徽医科大学药理教研室 合肥 230026，合肥 230026，合肥 230026，合肥 230032，合肥 230032，合肥 230032 |
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| 基金项目： | 国家博士后科学基金资助课题 |
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| 摘 要： | 利用带有积分球的SHIMADZU UV—240分光光度计,测量了组织在固定前和后的反射率和透射率,分析了福尔马林固定对组织光学性质的影响。
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| 关 键 词： | 组织 光学性质 固定 |
FORMALIN FIXATION'S INFLUENCE ON OPTICAL PROPERTIES OF TISSUES: |
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| Zhang Zhenxi Ma Yurong Fang Rongchuan.FORMALIN FIXATION''''S INFLUENCE ON OPTICAL PROPERTIES OF TISSUES:[J].ACTA Laser Biology Sinica,1993,2(1):204-206. |
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| Authors: | Zhang Zhenxi Ma Yurong Fang Rongchuan |
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| Abstract: | Before and after formalin fixation, we have got the characteristic reflectance and transmittance of the tissues in the wavelength regions of 200-900nm, using double-beam spectrometer and integrating sphere. we separately analyzed the influence of formalin fixation on optical reflectance and transmittance of tissues fixed by formalin. The results show that formalin fixation alters the optical properties of tissues.
The transmittance at 850nm of the tissue fixed by formalin increases at 850 nm from 31% to 45% as opposed to the tissue unfixed, but the reflectance at same wavelength decreases from 41% to 39%. we also noticed that changes of the Soret band ( approximately 413.6 nm ) , the a ( 541.0 nm ) and 3 ( 577 nm ) bands of oxyhemoglobin are remarkable |
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| Keywords: | Optical reflectance and transmittance of tissues Formalin fix-ation Biospectrometry Pathological diagnosis Integrating spheres Spectrum photometers |
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