激光辐照微藻的显微图像观察及荧光定量分析

利用激光共聚焦扫描显微镜观察激光辐照前后微藻细胞叶绿体自体荧光图像,并对荧光变化进行定量分析。用Nd:YAP激光辐照扁藻、金藻及三角褐指藻。实验结果表明:Nd:YAP激光辐照后,藻细胞荧光光谱峰位不变,但荧光峰值发生较大变化,在激光促长剂量辐照下,几种微藻细胞的荧光强度均比对照组强。激光辐照微藻产生的生理刺激效应可以反映在细胞的荧光特性与强度变化上。激光共聚焦扫描显微镜可以作为微藻激光生物效应研究的一种有效方法。

Observations and Analysis on Fluorescence of Microalgae Inducted by Laser

In this present paper,the Microalgae irradiated by laser are observed using Laser Confocal Scan Microscope,and the auto fluorescence of Microalgae cells is analyzed with quantitative analysis system,so as to investigate the effect of auto fluorescence alteration on the growth of irradiated Microalgae.Platymonas subcordiformis,Phaeodactylum tricormutum and Isochyrsis zhanjiangensis are irradiated by Nd:YAP laser.On contrast to the ck,the peak positions of fluorescence of irradiated algae cells is similar to the whole while the peak light intensity alters.On irradiation of promoting dose,however,the auto fluorescence intensity is enhanced more than control,with different type of Microalgae,the different fluorescence intensity are shown in the analysis,and all of the Microalgae pigment bodies change can be seen also.Physiological incentive effect of irradiated Microalgae can prompt the alteration to fluorescence characteristics and fluorescence intensity alteration of cells.