| 应用SCGE技术研究细胞DNA损伤的原理与方法 |
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| 引用本文： | 孟紫强,张连珍.应用SCGE技术研究细胞DNA损伤的原理与方法[J].中国生物化学与分子生物学报,1998,14(5):604-609. |
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| 作者姓名： | 孟紫强 张连珍 |
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| 作者单位： | 山西大学生命科学系(孟紫强)，中国辐射防护研究院二所(张连珍) |
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| 摘 要： | 对单细胞微凝胶电泳（ＳＣＧＥ）技术的操作过程，技术原理以及实验操作过程中应注意的事项，进行了详细介绍和讨论；并应用ＳＣＧＥ技术研究了γ射线照射对人血淋巴细胞ＤＮＡ的损伤效应．结果表明，γ射线照射能引起细胞ＤＮＡ迁移长度增加，且呈显著地剂量效应关系．
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| 关 键 词： | 单细胞微凝胶电泳 DNA损伤 淋巴细胞 电离辐射 |
| 收稿时间： | 1998-10-20 |
Principle and Method of the Single Cell Microgel Electrophoresis(SCGE) Technique to Study DNA Damage in Human Blood Lymphocytes |
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| Meng Zi,Qiang.Principle and Method of the Single Cell Microgel Electrophoresis(SCGE) Technique to Study DNA Damage in Human Blood Lymphocytes[J].Chinese Journal of Biochemistry and Molecular Biology,1998,14(5):604-609. |
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| Authors: | Meng Zi Qiang |
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| Institution: | (Division of Environmental Biological Toxicology and Department of Life sciences,Shanxi University,Taiyuan 030006) Zhang Lian Zhen (China Institute for Radiation Protection,Taiyuan 030006 |
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| Abstract: | The single cell microgel electrophoresis(SCGE)technique,its principle and noticeable details in the technique were described and disscussed.Effects of DNA damage in human blood lymphocytes induced by γ rays radiation were studied using this technique.It was shown that γ rays caused the increase of DNA migration length in the lymphocytes in the dose dependent manner. |
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| Keywords: | SCGE DNA damage Lymphocyte Ionizing radiation |
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