巴里坤湖和玛纳斯湖嗜盐菌的分离及功能酶的筛选

目的:了解新疆巴里坤湖与马纳斯湖中嗜盐菌及功能酶的多样性。方法:从两湖中采集水样进行菌种分离,采用PCR方法扩增出其16S rRNA基因(16S rDNA),并测定了基因的序列。对分离菌株进行了蛋白酶、淀粉酶、酯酶、脂肪酶、以及纤维素酶的筛选。结果:从两湖水样共分离得到51株嗜盐菌。基于16SrDNA序列的同源性比较和系统发育学分析,发现从两湖分离获得的中度嗜盐菌分别属于Planococcaceae、Bacillacea、Staphylococcus、Halomonadaceae、Salicolaceae以及Pseudomonadacaeae 6个属。分离得到的极端嗜盐古菌属于Halobacteriaceae属。功能酶筛选结果表明产蛋白酶的嗜盐菌共有15株,产酯酶的共有23株,产淀粉酶的共有8株,未获得产脂肪酶和纤维素酶的嗜盐菌。结论:新疆巴里坤湖和马纳斯湖中有丰富的嗜盐微生物资源及酶资源,有重要的研究意义和应用前景。

Isolation and Enzyme Screening of Halophiles from Barkol Lake and Manasi Lake

Objective:To understand the diversity of halophilic archaea and bacteria diversity and the extracellular hydrolytic enzymes diversity of Barkol lake and Manasi lake. Methods: Water samples were collected and the phylogenetic diversity of halophilic bacteria associated with the water was investigated. Protease, easterase, amylase, lipase and cellulase from these strains were screened. Results: 51 strains of halophilic bacteria were isolated. On the basis of the homology and phylogenetic analysis of 16S rDNA, it was considered that the halophilic bacteria were identified as members of halophilic bacteria from the two lakes were related to the genera Planococcaceae, Bacillaceae, Staphylococcus, halomonadaceae, Salicolaceae, Pseudomonadacaeae and extremely halophilic archaea strains were identified as members of the generaHalobacteriaceae. 15 strains produce protease, 23 strain produce esterase, 8 strains produce amylase and no strain produce lipase and cellulase. Conclusion: It was indicated that there are abundant resource of halophilic microorganism and enzyme in Barkol lake and Manasi lake.