| 应用cDNA芯片分析79个新基因的人胚组织表达谱 |
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| 引用本文： | 马淑华,王敦成,邹宗亮,沈倍奋,王升启.应用cDNA芯片分析79个新基因的人胚组织表达谱[J].生物化学与生物物理进展,2001,28(4):532-536. |
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| 作者姓名： | 马淑华 王敦成 邹宗亮 沈倍奋 王升启 |
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| 作者单位： | 1. 军事医学科学院放射医学研究所,
2. 军事医学科学院基础医学研究所, |
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| 基金项目： | 国家自然科学基金重点项目(39889001). |
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| 摘 要： | 大规模cDNA测序和生物信息学技术相结合，得到来自于商品化的人胚肾cDNA文库79个代表新基因的表达序列标签(EST).随后，采用高速度机械手制备这些cDNA的基因芯片，用于鉴定79个新基因的ESTs在20周、26周两个胚胎时期6种组织中的基因表达状况，以研究这些EST片段代表的新基因功能提供线索.通过芯片杂交及结果分析，得到同一个组织两个不同时相8个差异表达的基因，随后的RNA印迹分析的结果与芯片杂交的结果相一致.
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| 关 键 词： | cDNA芯片，新基因，表达序列标签（EST），差异表达RNA分析 |
| 收稿时间： | 2000/10/8 0:00:00 |
| 修稿时间： | 2000年10月8日 |
The 79 Novel Genes Expression Pattern in Human Fetal Tissues was Monitored by Using cDNA Microarray |
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| MA Shu-Hu,WANG Dun-Cheng,ZOU Zong-Liang,SHEN Bei-Fen and WANG Sheng-Qi.The 79 Novel Genes Expression Pattern in Human Fetal Tissues was Monitored by Using cDNA Microarray[J].Progress In Biochemistry and Biophysics,2001,28(4):532-536. |
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| Authors: | MA Shu-Hu WANG Dun-Cheng ZOU Zong-Liang SHEN Bei-Fen and WANG Sheng-Qi |
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| Institution: | Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract: | 79 EST fragments which represents corresponding novel genes were obtained by sequencing and bioinformatics analysis of human fetal kidney cDNA library. Microarray was prepared by using these novel EST fragments by automatic spotting. Expression patters of 79 ESTs of novel genes from human fetal kidney were analyzed in fetal brain and fetal heart tissues of 20-week-age and 26-week-age fetus by performing of cDNA chip hybridization. This provides clues for studying exact functions of the novel genes. 8 genes were obtained which were expressed differentially in the fetal brain and heart of 20-week- and 26-week-age respectively. Then differentially expressed genes were identified by Northern analysis. The more exact function of the novel genes are under study. |
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| Keywords: | cDNA chip novel genes expressed sequence tag (EST) differential expression Northern analysis |
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