组织工程静脉血管种子细胞的培养

目的获取可应用于静脉血管组织工程的种子细胞内皮细胞。方法选取北京地区雄性杂种犬,取其双侧颈外静脉,采用翻转后酶消法,获取原代内皮细胞,对其进行原代培养和传代培养,冻存、复苏和鉴定,并利用光学显微镜进行观察。结果获取犬的颈外静脉后,实验采取了翻转后酶消法,所获得的内皮细胞纯度和数量得到了提高;经过鉴定确实为内皮细胞来源;活性好,增殖快,在较短的时间内能够达到后期实验所需数量。结论经体外培养的犬的颈外静脉内皮细胞可以作为组织工程血管的种子细胞。

Culture of Venous Endothelial Cells for Blood Vessel Tissue Engineering

Objective To obtain venous endothelial cells to be used in the research of blood vessel tissue engineering.Methods The external jugular vein of canine was resected under aseptic conditions.The primary endothelial cells were harvested,cultured,identified and multiplicated,and observed by optical microscopy.Result The endothelial cells of the canine external jugular vein,cultured and multiplicated in vitro,not only had good bioactivity,but also met the need of experiments in amount.Conclution Endothelial cells of canine external jugular vein cultured in vitro may fit the need of blood vessel tissue engineering.