Effects of green LED light and three stresses on biomass and lipid accumulation with two-phase culture of microalgae |
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Institution: | 1. Algae R&D Centre, School of Veterinary and Life Sciences, Murdoch University, Murdoch, 6150 Western Australia, Australia;2. School of Engineering and Information Technology, Physics and Energy, Murdoch University, Murdoch, 6150 Western Australia, Australia |
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Abstract: | The effects of wavelengths of light-emitting diode (LED), nitrate concentration, and salt concentration were evaluated for the two-phase culture of the microalgal species Phaeodactylum tricornutum, Dunaliella tertiolecta, and Isochrysis galbana on cell growth and lipid production. Blue LEDs produced the highest biomass of P. tricornutum at a nitrate concentration of 8 mg/L, reaching 0.97 g dcw/L with a specific growth rate (μ) of 0.047 h?1, followed by I. galbana with 0.79 g dcw/L and μ = 0.040 h?1 and D. tertiolecta with 0.55 g dcw/L and μ = 0.028 h?1. Of the three microalgae, P. tricornutum had the highest specific growth rate of μmax = 0.070 h?1 and lowest saturation constant of Ks = 4.18 mg/L, resulting in fast cell growth. The highest lipid production was obtained under green LED wavelength stress on day 14, reaching 60.6% (w/w) of the dry cell weight among the three microalgae. The main fatty acids produced by the three microalgae were myristic acid (C14:0), palmitic acid (C16:0), oleic acid (C18:1), and arachidic acid (C20:0), which comprised 72.68%–84.16% (w/w) of the total fatty acids content under three stresses. |
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Keywords: | Microalgae Nitrate depletion Salt stress Green LED wavelength stress Fatty acid profiles |
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