| 小麦高分子量麦谷蛋白5亚基基因的克隆及其表达载体的构建 |
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| 引用本文： | 陈新建,吕德彬,陈军营,陈占宽,程西永.小麦高分子量麦谷蛋白5亚基基因的克隆及其表达载体的构建[J].植物生理与分子生物学学报,2003,29(2):165-168. |
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| 作者姓名： | 陈新建 吕德彬 陈军营 陈占宽 程西永 |
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| 作者单位： | 1. 河南农业大学农学院,郑州,450002
2. 河南农业科学院农作物新品种重点实验室,郑州,450002 |
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| 基金项目： | theChineseNationalSpecialFoundationforTransgenicPlantResearchandCommercialization (No .J0 0B0 2 0 )andtheHenan
ProvinceOutstandingIndividualInnovationFoundation (No .
0 2 2 10 0 0 90 0 ) |
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| 摘 要： | 小麦麦谷蛋白5亚基直接影响面包的烘烤品质,但我国大部分小麦品种的蛋白中缺少这种亚基。通过引物设计、PCR扩增,从Cheyenne中得到了小麦麦谷蛋白5亚基结构基因(sub5)的全长核苷酸序列(2750bp)和小麦麦谷蛋白5亚基基因启动子(Psub5)的核酸序列(630bp)。测序结果表明：得到了小麦籽粒中特异表达启动子——Psub2和用于改良小麦品质的结构基因——sub5。通过选择和改变相应的酶切位点,在构建6个中间载体的基础上,最后得到了含有目的基因的表达载体pCAM—BIAl301—Psub5—sub5—nos。酶切电泳及PCR鉴定表明：已成功地合成了sub5的表达载体。有希望通过基因工程的方法将该表达载体用于小麦的品质改良。
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| 关 键 词： | 小麦 Glu－1D－1d基因 克隆 |
| 修稿时间： | 2002年1月10日 |
Cloning of Wheat High Molecular Weight Glutenin Subunit 5 Gene and Construction of Its Expression Vector |
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| CHEN Xin-jian,LU De-Bing,CHEN Jun-Ying,CHEN Zhan-Kuan,CHENG Xi-yong.Cloning of Wheat High Molecular Weight Glutenin Subunit 5 Gene and Construction of Its Expression Vector[J].Journal Of Plant Physiology and Molecular Biology,2003,29(2):165-168. |
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| Authors: | CHEN Xin-jian LU De-Bing CHEN Jun-Ying CHEN Zhan-Kuan CHENG Xi-yong |
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| Abstract: | Using the PCR amplification technology, a full-length nucleotide sequence (2 750 bp) of wheat high molecular weight glutenin subunit 5 (or Dx5) gene(Glu-1D-1d) and its promoter sequence (630 bp), were cloned from "Cheyenne" wheat. By the selection and reparation of corresponding sites of restriction enzymes, a chimaeric gene, comprising the promoter (P sub5), the structure gene(Glu-1D-1d) and the nopaline synthase (Nos) gene terminator, i.e. pCAMBIA1301-P HGBy9-sub5-nos, was successfully constructed and verified after five transitional plasmids being produced. |
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| Keywords: | wheat Glu-1D-1d gene clone |
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