棉铃虫田间种群抗药性的生态学干扰和生化机制研究

从生态遗传学角度和抗性机制两个方面研究了作物布局和解毒酶系对棉铃虫高效氯氰菊酯抗药性的影响，通过田间采样，测定了姜堰，如皋，兴化3个地区的棉铃虫对高效氯氰菊酯的抗药性。结果表明，作物布局对棉铃虫菊酯抗性的发展具有明显的影响，寄主相对单一的棉花连片种植区（兴化）棉田棉铃虫对高效氯氰菊酯的抗性水平最高，是棉花春玉米混栽区（如皋）棉田棉铃虫抗药性的3．5倍，在邻近春玉米种植区的稻棉区（姜堰），棉田棉铃虫的抗药性水平，是4－5km外玉米田棉铃虫的4．8倍，研究同时表明，四代棉铃虫的抗药性比3代棉铃虫的抗药性下降了2．3倍，棉铃虫离体解毒酶和乙酰胆碱酯酶活性测定表明，棉铃虫的羧酸酯酶，谷胱甘肽S－转移酶和乙酰胆碱酯酶活性与棉铃虫对高效氯氰菊酯的抗药性水平有显著的相关性，对姜堰南部棉田和如振戴庄棉田棉铃虫的活体增效试验表明，多功能氧化酶是抗性棉铃虫对高效氯氰菊酯重要的解毒酶系，增效醚可分别增效22．79倍和12．33倍，羧酸酯酶对抗性棉铃虫的解毒代谢也有部分贡献，磷酸三苯酯可分别增效4．41倍和3．70倍。

Studies on the ecological disturbance on resistance of Helicoverpa armigera and biochemical mechanism

The cotton bollworm Heliothis Armigera is a serious pest of cotton and other economic crops in China,where it has developed resistance to several kinds of insecticides,such as pyrethroids,cabamates and organophosphates,and has caused huge economic loss and even catastrophe to cotton production.In turn,the cotton bollworm has received much attention in the past decade in toxicological studies.However,classic research on resistant cotton bollworm is mainly limited to the laboratory study,little attention has...