多器官功能不全综合征时肠道细菌微生态学改变的实验研究

为研究多器官功能不全综合征（ＭＯＤＳ）时肠道细菌微生态的变化以及Ｇ＾－杆菌消长与肠道和血液中内毒素水平的关系，本实验选用ＳＤ大鼠，应用无菌Ｚｙｍｏｓａｎ腹腔注射制备大鼠ＭＯＤＳ模型，并对ＭＯＤＳ大鼠肠道菌群进行定量分析，对门静脉和外周静脉血中的内毒以及肠道游离内毒素含量进行定量测定。结果发现，ＭＯＤＳ状态下肠道细菌微生态发生明显变化，表现为肠杆菌和肠球菌等肠道内需氧菌的数量明显增多，双歧杆菌和乳酸

THE DISTURBANCE OF INTESTINAL MICROECOLOGY IN MODS MODELS

To investigate changes of intestinal microecology and its relationships with contents of endotoxin in the portal and peripheral blood as well as the intestinal tract, the MODS model was prepared by intraperitoneal injection of Zymosan A, and the intestinal microflora was analysed quantitatively, the contents of endotoxin in the portal and peripheral blood as well as the intestinal tract were measured. The results showed: 1) there were significant changes in intestinal microflora in the state of MODS, the number of aerobic enterobacteriaceae and enterococci were increased, the number of obligate anaerobic Bifidobacteria and Lactobacillus decreased markedly; 2) with the changing of the intestinal microflora, the number of gram negtive bacteria (GNB) incrased, the endotoxin pool expanded and the levels of endotoxin in peripheral and portal blood increased. It was suggested that there were disturbance of intestinal microecology in the MODS developing process, and proliferation of GNB, intestinal free endotoxin and endotoxemia during MODS was in association with the disturbance of intestinal microecology.