Molecular characterization of S-RNase genes and S-genotypes in the Japanese apricot (Prunus mume Sieb. et Zucc.) |
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Authors: | H Yaegaki T Shimada T Moriguchi H Hayama T Haji M Yamaguchi |
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Institution: | (1) National Institute of Fruit Tree Science, Tsukuba, Ibaraki 305-8605, Japan e-mail: yaegaki@fruit.affrc.go.jp Fax: +81-298-38-6437, JP;(2) National Institute of Fruit Tree Science (Okitsu), Shimizu, Shizuoka 424–0292, Japan, JP |
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Abstract: | Three partial S-RNase genes, MSRN-1, MSRN-2, and MSRN-3, in the Japanese apricot (Prunus
mume Sieb. et Zucc.) were isolated from the three cultivars Nankou, Gyokuei, and Kairyouuchidaume, respectively. The structural
characteristics revealed that S-RNase genes from the Japanese apricot were in the T2/SRNase-type S-RNase family with five conserved regions (C1, C2, C3, RC4, and C5) and one variable region (RHV) as reported in the other
rosaceous plants. In the phylogenetic tree of T2/S SRNase-type RNases, three S-RNase genes of the Japanese apricot did not form a species-specific subgroup but the Prunus subfamily did. At least seven S-allelic genes were present in the Japanese apricot, and S-genotypes of six representative cultivars, including Nankou, Gyokuei, Kairyouuchidaume, Baigou, Kagajizou, and Oushuku were
first established in this study as S
1
S
7, S
2
S
6, S
3
S
4, S
3
S
6, S
3
S
6 and S
1
S
5, respectively. An extended elucidation of the S-genotype would contribute to a more efficient breeding program of the Japanese apricot.
Received: 5 September 2000 / Revision accepted: 22 December 2000 |
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Keywords: | Gametophytic self-incompatibility Japanese apricot Molecular identification S-genotype S-RNase |
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