| 紫萍叶状体衰老过程中的内肽酶谱变化和外源L-丝氨酸对内肽酶谱的影响 |
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| 引用本文： | 刘清岱,朱晔荣,李洪涛,王勇.紫萍叶状体衰老过程中的内肽酶谱变化和外源L-丝氨酸对内肽酶谱的影响[J].植物生理学通讯,2010(3):241-244. |
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| 作者姓名： | 刘清岱 朱晔荣 李洪涛 王勇 |
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| 作者单位： | [1]天津科技大学食品工程与生物技术学院,天津300457 [2]南开大学生命科学学院,天津300071 |
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| 基金项目： | 国家自然科学基金（30870185）. |
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| 摘 要： | 采用双向凝胶电泳技术分析紫萍叶状体衰老期间内肽酶同工酶的变化以及外源L-丝氨酸对内肽酶影响的结果表明，在衰老的紫萍叶状体中检测到9种内肽酶同工酶，丝氨酸内肽酶EP3可能在叶状体衰老的早期起作用，而半胱氨酸内肽酶EP9在第6天出现，是衰老后期活性最强的内肽酶。培养液中加入外源的L-丝氨酸后，叶状体蛋白质含量下降加剧，与衰老相关的内肽酶EP3、EP4和EP9的活性明显增强或提前出现。
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| 关 键 词： | 紫萍 叶状体 衰老 内肽酶 L-丝氨酸 |
Changes of Endopeptidase Isoenzymes in Spirodela polyrrhiza Thallus during Senescence and Effect of Exogenous L-Serine on Endopeptidase Isoenzymes |
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| LIU Qing-Dai,ZHU Ye-Rong,LI Hong-Tao,WANG Yong.Changes of Endopeptidase Isoenzymes in Spirodela polyrrhiza Thallus during Senescence and Effect of Exogenous L-Serine on Endopeptidase Isoenzymes[J].Plant Physiology Communications,2010(3):241-244. |
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| Authors: | LIU Qing-Dai ZHU Ye-Rong LI Hong-Tao WANG Yong |
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| Institution: | 1College of Food Engineering and Biotechnology, T[anjin University of Science and Technology, Iianjin 300457, China; 2College of Life Sciences, Nankai University, Tianjin 300071, China |
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| Abstract: | Changes in endopeptidase isoenzymes of Spirodela polyrrhiza thallus during senescence and L-serine induced senescence were investigated by two-dimensional electrophoresis. Nine endopeptidases were detected. Changes of these enzymes activities during senescence showed that EP3 （a serine protease） might play a significant role in early stage and the EP9 （a cysteine protease）, appeared on the 6th day, was the most active endopeptidase in the late stages of senescence. L-serine treatment of the thallus enhanced the appearance and increase in the activities of three senescence-related endopeptidases （EP3, EP4, EP9）, along with its significant promotion of decline in protein content. |
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| Keywords: | Spirodela polyrrhiza thallus senescence endopeptidase L-serine |
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