肝细胞癌患者外周血HSF1 基因表达的检测及应用价值

目的:建立检测HSF1 mRNA的real-time PCR的方法,了解肝细胞癌患者外周血中HSF1的表达水平及其与各临床病理特征之间的关系。方法:利用real-time PCR的方法检测20例肝细胞癌患者及20例正常人群外周血中HSF1 mRNA的表达量。结果:肝细胞癌患者外周血中的HSF1 mRNA表达量显著高于正常人群(P<0.05);肝细胞癌患者外周血中HSF1 mRNA的表达水平在不同性别、肿瘤大小、门静脉侵犯情况、HbsAg水平及AFP水平的患者中的差异无统计学意义(P>0.05);在不同病理分化程度、TNM分期的患者中的差异有统计学意义(P<0.05)。结论:real-time PCR技术可以成功检测外周血中HSF1 mRNA的表达量,HSF1可能与肝细胞癌的发生发展密切相关。

Development and Application of Real-time Quantitative Polymerase Chain Reaction for the Detection of Human Hepatocellular Carcinoma Related Novel Gene HSF1 Expression

Objective: To establish a quantitative real-time PCR method for detection of HSF1 gene expression level in human hepatocellular carcinoma and to investigate the relationship between HSF1 mRNA expression level and the clinicopathological characters.Methods: Real-time PCR was employed to determine the expression level of HSF1 mRNA in peripheral blood of 20 specimens of human hepatocellular carcinoma patients and normal human.Results: Comparing with normal human,HSF1 present higher expressions in human hepatocellular carcinoma patients(P<0.05).There is no statistical differences of HSF1 mRNA expression level between the group with different sexes,tumour diameters,portal involvement levels,HBsAg levels and AFP levels(P>0.05).There is statistical differences of HSF1 mRNA expression level between the group with different differentiations and TNM stages(P>0.05).Conclusion: The established quantitative real-time PCR method can successfully detect the expression level of HSF1 mRNA,which may be closely related to the occurrence and development of human hepatocellular carcinoma.