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| 多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究 | |
| 引用本文： | 范磊,张道敬,刘振华,陶黎明,罗远婵,魏鸿刚,李淑兰,李元广.多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究[J].天然产物研究与开发,2012,24(6):729-735. |
| 作者姓名： | 范磊 张道敬 刘振华 陶黎明 罗远婵 魏鸿刚 李淑兰 李元广 |
| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室 2. 华东理工大学药学院 3. 上海泽元海洋生物技术有限公司,上海,200237 |
| 基金项目： | 国家农业科技成果转化资金项目，国家863项目，上海市重点学科建设项目 |
| 摘 要： | 对一株已经商业化的可防治植物枯萎病的生防菌-多粘类芽孢杆菌HY96-2发酵液中抗真菌活性物质进行了分离纯化,采用酸化、正丁醇萃取、乙酸乙酯沉淀、硅胶柱层析、Sephadex LH-20柱层析、高效液相色谱(HPLC)等方法分离得到了一个抗真菌活性化合物6B,经NMR、MS、MS/MS等光谱学方法鉴定其为Fusaricidin A。琼脂扩散法抑菌试验结果表明,6B对西瓜枯萎病菌、水稻纹枯病菌、灰霉病菌等15种植物病原真菌的最小抑菌浓度为12.5～50μg/mL。盆栽实验结果表明,6B对黄瓜灰霉病具有明显的防治效果,当6B的浓度达到250μg/mL时,防治效果高达95%。 |
| 关 键 词： | 多粘类芽孢杆菌 抗真菌活性 分离纯化 结构鉴定 Fusaricidin A |
Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2 |
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| FAN Lei , ZHANG Dao-jing , LIU Zhen-hua , TAO Li-ming , LUO Yuan-chan , WEI Hong-gang , LI Shu-lan , LI Yuan-guang.Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2[J].Natural Product Research and Development,2012,24(6):729-735. | |
| Authors: | FAN Lei ZHANG Dao-jing LIU Zhen-hua TAO Li-ming LUO Yuan-chan WEI Hong-gang LI Shu-lan LI Yuan-guang |
| Institution: | 1* 1State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology;2School of Pharmacy, East China University of Science and Technology;3Shanghai Zeyuan Marine Biotechnology Co.Ltd.,Shanghai 200237,China |
| Abstract: | Isolation and purification of antifungal substance from Paenibacillus polymyxa HY96-2,a commercial biocontrol strain used in control of fusarium wilt disease,was studied in this paper.By means of acidification,n-BuOH extraction,ethyl acetate precipitation,silica gel column chromatography,sephadex LH-20 column chromatography and HPLC preparation,6B was isolated and purified.Its structure was elucidated as fusaricidin A by means of spectroscopic analysis including NMR,MS and ESI-MS/MS.Results of two-fold serial agar dilution method showed that the minimum inhibition concentrations of 6B against 15 phytopathogenic fungi were 12.5-50 μg/mL.Greenhouse results showed that 6B had better control effect on Botrytis cinerea of cucumber.The control effect could reach 95% when its concentration was set at 250 μg/mL. |
| Keywords: | Paenibacillus polymyxa antifungal activity isolation and purification structure identification fusaricidin A |
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