| 巴西固氮螺菌Yu62的EGFP标记及其在小麦体内的定殖研究 |
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| 引用本文： | 刘华伟,王庆贺,张宏,王蕊,肖红利,郭蔼光.巴西固氮螺菌Yu62的EGFP标记及其在小麦体内的定殖研究[J].西北植物学报,2009,29(12). |
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| 作者姓名： | 刘华伟 王庆贺 张宏 王蕊 肖红利 郭蔼光 |
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| 作者单位： | 西北农林科技大学,生命科学学院,陕西杨陵,712100 |
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| 摘 要： | 以质粒pEGFP-C1为模板,采用PCR方法特异性扩增增强型绿色荧光蛋白(EGFP)基因全长序列,将其与原核表达载体pVK-100连接,构建成重组载体pVK-EGFP.利用电转化法将重组载体导入巴西固氮螺菌Yu62中,得到EGFP标记菌株.用EGFP标记菌接种小麦'小偃107'种子,室内限菌条件下培养10 d后,用荧光显微镜观测标记菌在小麦体内的定殖规律并观察接菌植株的田间生长状况.结果显示,巴西固氮螺菌Yu62能定殖于小麦根毛区、茎组织的细胞间隙等部位,而且接菌小麦'小偃107'植株在根系发育、株高、分蘖数等方面比对照有较明显的优势.研究表明,巴西固氮螺菌Yu62能够定殖于小麦根茎内,并具有促进植物生长的作用.
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| 关 键 词： | 增强型绿色荧光蛋白(EGFP) 巴西固氮螺菌Yu62 小麦 定殖 |
Colonization of Azospirillum brasilense Yu62 in Wheat Via EGFP |
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| LIU Hua-wei,WANG Qing-he,ZHANG Hong,WANG Rui,XIAO Hong-li,GUO Ai-guang.Colonization of Azospirillum brasilense Yu62 in Wheat Via EGFP[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(12). |
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| Authors: | LIU Hua-wei WANG Qing-he ZHANG Hong WANG Rui XIAO Hong-li GUO Ai-guang |
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| Institution: | LIU Hua-wei,WANG Qing-he,ZHANG Hong,WANG Rui,XIAO Hong-li,GUO Ai-guang(College of Life Science,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The full-length gene encoding the enhanced green fluorescent protein(EGFP) was amplified via PCR reaction by using the prokaryotic expression vector pEGFP-C1 as the template,followed by ligation with plasmid pVK-100 whereupon a recombinant plasmid pVK-EGFP was constructed.The plasmid was transferred into the strain Azospirillum brasilense Yu62 by electroporation.A breed,'Xiaoyan 107' were inoculated with the labeled strain and then the pattern of colonization and the growth of the wheat was observed with in... |
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| Keywords: | enhanced green fluorescent protein (EGFP) Azospirillum brasilense Yu62 wheat colonization |
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