Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid |
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Authors: | Yuriko Kurioka Yoshihito Suzuki Hiroshi Kamada Hiroshi Harada |
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Institution: | (1) Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305 Ibaraki, Japan;(2) Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan |
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Abstract: | Summary Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of the rolC gene.Abbreviations native rolC
rolC gene under control of its own promoter
- 35S-rolC
rolC gene under control of a cauliflower mosaic viras 35S promoter |
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Keywords: | Atropa belladonna Flowering Ri plasmid rolC gene Transformation |
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