AFLP analysis of variation in pecan somatic embryos |
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Authors: | W A Vendrame G Kochert H Y Wetzstein |
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Institution: | (1) Department of Horticulture, University of Georgia, Plant Sciences Bldg., Athens, GA 30602-7273, USA Fax: 706-542-0624 e-mail: hywetz@arches.uga.edu, GE;(2) Department of Botany, University of Georgia, Plant Sciences Bldg., Athens, GA 30602-7271, USA, GE |
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Abstract: | Before somatic embryogenesis can be applied, the genetic fidelity of cultures needs to be determined. Problematic of tissue-cultured
woody species is the extensive evaluation time needed for assessments. The development of methods whereby plants could be
rapidly screened for potential tissue culture-derived genetic changes would be very valuable. We evaluated the applicability
of AFLP (amplified fragment length polymorphism) analysis for the assessment of genetic variability in somatic embryos of
pecan Carya illinoinensis (Wangenh.) C. Koch] and made comparisons between and within embryogenic culture lines. AFLP readily detected differences
between culture lines, with 368 polymorphic loci identified. Individual culture lines generally produced somatic embryos with
similar overall banding patterns. Embryos derived from the same culture line generally grouped together in a phenogram generated
by UPGMA (unweighted pair-group method, arithmetic average) analysis. However, a few somatic embryos exhibited higher levels
of polymorphism and failed to group with others regenerated from the same line. The relation between the detected within-line
differences and their contribution to phenotypic variation is yet to be determined.
Received: 9 September 1998 / Revision received: 12 November 1998 / Accepted: 8 December 1998 |
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Keywords: | Carya illinoinensis Somatic embryogenesis AFLP Genetic fidelity |
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