Rhizobium indicum sp. nov., isolated from root nodules of pea (Pisum sativum) cultivated in the Indian trans-Himalayas |
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| Institution: | 1. National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra 411007, India;2. Department of Biology, Queen''s University, Kingston, K7L 3N6, Canada;3. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India;1. Laboratory of Biodiversity and Valorization of Arid Areas Bioresources (BVBAA) – Faculty of Sciences of Gabes, Erriadh, Zrig 6072, Tunisia;2. Centro de Biotecnología y Genómica de Plantas (UPM-INIA), ETSI Agronómica, Alimentaria y de Biosistemas, Campus de Montegancedo, Universidad Politécnica de Madrid, Madrid, Spain;1. Embrapa Soja, C.P. 231, 86001-970 Londrina, Paraná, Brazil;2. Departamento de Microbiologia, Universidade Estadual de Londrina, C.P. 10011, 86057-970 Londrina, Paraná, Brazil;3. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, SBN, Quadra 2, Bloco L, Lote 06, Edifício Capes, 70.040-020 Brasília, Distrito Federal, Brazil;4. Conselho Nacional de Desenvolvimento Científico e Tecnológico, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, 71605-001 Brasília, Distrito Federal, Brazil;5. Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Avenida General Carlos Cavalcanti, 4748 – Uvaranas, C.P. 6001, Ponta Grossa, PR 84030?900, Brazil;1. Research Laboratory Biodiversity and Valorization of Arid Areas Bioresources (BVBAA) – Faculty of Sciences of Gabès, Erriadh, 6072, Tunisia;2. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, Pozuelo de Alarcón, Madrid, 28223, Spain;3. Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, Madrid, 28040, Spain;4. Instituto de Ciencias Agrarias, CSIC, Madrid, 28006, Spain;1. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid — Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28223 Pozuelo de Alarcón, Madrid, Spain;2. Microbiologia, Departament de Biologia, Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain;3. Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, 28006 Madrid, Spain;1. Centre de Biotechnologies Végétales et Microbiennes, Biodiversité et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Morocco;2. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), E-419, 18080 Granada, Spain;1. Laboratory of General and Agricultural Microbiology, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece;2. Laboratory of Vegetable Production, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece |
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| Abstract: | Three strains of rhizobia isolated from effective root nodules of pea (Pisum sativum L.) collected from the Indian trans-Himalayas were characterized using 16S rRNA, atpD and recA genes. Phylogeny of the 16S rRNA genes revealed that the newly isolated strains were members of the genus Rhizobium with ≥99.9% sequence similarity to the members within the “Rhizobium leguminosarum” group. Phylogenetic analyses based on the concatenated sequences of atpD and recA gene, and 92 core genes extracted from the genome sequences indicated that strains JKLM 12A2T and JKLM 13E are grouped as a separate clade closely related to R. laguerreae FB206T. In contrast, the strain JKLM 19E was placed with “R. hidalgonense” FH14T. Whole-genome average nucleotide identity (ANI) values were 97.6% within strains JKLM 12A2T and JKLM 13E, and less than 94% with closely related species. The digital DNA-DNA hybridization (dDDH) values were 81.45 within the two strains and less than 54.8% to closely related species. The major cellular fatty acids were C18:1w7c in summed feature 8, C14:0 3OH/C16:1 iso I in summed feature 2, and C18:0. The DNA G + C content of JKLM 12A2T and JKLM 13E was 60.8 mol%. The data on genomic, chemotaxonomic, and phenotypic characteristics indicates that the strains JKLM 12A2T and JKLM 13E represent a novel species, Rhizobium indicum sp. nov. The type strain is JKLM 12A2T (= MCC 3961T = KACC 21380T = JCM 33658T). However, the strain JKLM 19E represents a member of “R. hidalgonense” and the symbiovar viciae. |
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| Keywords: | Rhizobia Symbiosis Nitrogen fixation High altitude agriculture nifH gene nodC gene |
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