毛头鬼伞(Coprinus comatus)中一种碱性蛋白的纯化及其活性

用离子交换层析(CM-sepharose FF)和凝胶层析(Superdex^TM 75)方法，从新鲜食用菌毛头鬼伞(Coprinus omatus)子实体中分离纯化出一碱性蛋白y3，经SDS-PAGE初步确定其分子量约为14．4kD。活性检测结果显示：当其浓度为12．5μg／mL时，对烟草花叶病毒(TMV)在心叶烟枯斑寄主上的侵染抑制率达83．0%；y3对兔血凝集活性滴度为2^5，对人血凝集活性滴度为26，其浓度分别为1．562μg／mL和0．78lμg／mL；利用胃癌细胞株MGC-803检测y3体外抗肿瘤活性，其IC50为12μg／mL。y3 N-端序列为NRDVAACARFIDDFCDTLTP，为一新的蛋白序列。在SWISS-PORT上登录号为P83477。

Purification and Activities of an Alkaline Protein from Mushroom Coprinus comatus

An Alkaline protein, y3, can be purified from the fruiting bodies of mushroom Coprinus comatus by means of CM-sepharose FF ion-exchange column chromatography and Superdex 75 High Resolution molecular sieve chromatography. The protein has a molecular weight of about 14.4kD by SDS-PAGA. Some activities of y3 have been detected, and the result is the following: the inhibition rate against Tobacco Mosaic Virus is 83.0% when the concentration of y3 is 12.5 microg/ mL. y3 is able to agglutinate rabbit and human erythrocytes at the concentration of 1.562 microg/mL and 0.781 microg/mL. Using an assay system based on mach cancer cell line MGC-803, y3 was studied for its inhibitory ability against celles multiplication, and the IC50 is 12 microg/mL. The N-terminal sequence is NRDVAACARFIDDFCDTLTP, which has no homology with other sequences in Genbank.