Influence of triacontanol on somatic embryogenesis in <Emphasis Type="Italic">Coffea arabica</Emphasis> L. and <Emphasis Type="Italic">Coffea canephora</Emphasis> P. ex Fr.

Summary A highly reproducible method for regeneration of Coffea arabica and C. canephora plants via direct somatic embryogenesis from cultured leaf and stem segments of regenerated plants was developed. Embryogenesis was influenced by the presence of triacontanol (TRIA) in the medium. TRIA incorporated at 4.55 and 11.38 μM in half-strength MS basal medium containing 1.1 μM 6-benzyladenine (BA) and 2.28 μM indole-3-acetic acid (IAA) induced direct somatic embryogenesis in both species. A maximum of 260±31.8 and 59.2±12.8 somatic embryos per culture were induced from in vitro leaf explants of C. arabica and C. canephora, respectively. TRIA also induced embryo formation from in vitro stem segment callus tissues along with multiplication of primary embryos into secondary embryos. By using TRIA, it was possible to obtain somatic embryogenesis in C. arabica and C. canephora.     

Triacontanol: a potent plant growth regulator in agriculture

Abstract

Triacontanol (TRIA) is a natural plant growth regulator found in epicuticular waxes. It is used to enhance the crop production in millions of hectares, particularly in Asia. Quite a number of researchers have reported the TRIA-mediated improvement in growth, yield, photosynthesis, protein synthesis, uptake of water and nutrients, nitrogen-fixation, enzymes activities and contents of free amino acids, reducing sugars, soluble protein, and active constituents of essential oil in various crops. Expectedly, TRIA enhances the physiological efficiency of the cells and, thus, exploits the genetic potential of plant to a large extent. In fact, TRIA increased free amino acids, reducing sugars, and soluble protein of rice (Oryza sativa L.) and maize (Zea mays L.) within 5 min. TRIA elicited the appearance of L(+)-adenosine within 1 min in the roots of plants, the shoots of which were sprayed with nanomolar concentrations of TRIA. TRIA and octacosanol (OCTA), the primary alcohols, are ubiquitous in the environment. OCTA was reported to inhibit the activity of TRIA in the seedlings of rice (Oryza sativa L.) at equimolar concentrations; and both TRIA and OCTA elicited a second messenger, known as OCTAM and triacontanol second messenger (TRIM), respectively. TRIA rapidly increases the ratio of L(+)- to D(–)-adenosine, probably at the tonoplast. However, it is to be resolved as to how TRIA elicits L(+)-adenosine and what is the source of L(+)-adenosine in plants. Based on known metabolic processes, de novo synthesis of L(+)-adenosine is unlikely, because of the rapidity of the response. TRIA-mediated increase in dry matter production could influence the inter-relationship between primary and secondary metabolism, leading to increased biosynthesis of secondary products. Various studies present strong evidences that application of TRIA applied either to the root medium or to leaves enhanced the growth and yield of vegetables and other crops, including agronomic and horticultural crops as well as medicinal and aromatic crop plants under normal and adverse conditions. However, further investigations are required to elucidate the possible role of TRIA on plant growth regulation, physiological activities and secondary metabolite biosynthesis regarding medicinal and aromatic plants subjected to abiotic stress. The present review covers the pivotal role of TRIA in plant growth and development, its mode of action and its significance in improving the crop productivity and quality of agricultural crops.     

Effects of Tomatex /Triacontanol/ on chlorophyll fluorescence and tomato (Lycopersicon esculentum Mill.) yields

The influence of triacontanol in a form of Tomatex preparation on basic indices of chlorophyll fluorescence in tomato leaves (Delfina cv.), yield of fruits, and dry matter content in fruits was evaluated in a pot experiment situated in vegetation hall in 1999. Tomatex was applied into roots at seedling stage (6–7 leaves) or at the stage of seedling and flowering of the 2^nd inflorescence bunch. Plants were given by 0.3, 3.0, and 30 μg triacontanol per pot at a single dosage. Results obtained have shown that triacontanol regardless of the dose applied, significantly increased the maximal efficiency of PSII photochemistry in the dark (F_v/Fm), the efficiency of excitation capture by open PSII reaction centers (F_v’/F_m’), the actual quantum yield of PSII electron transport in the light-adapted state (Φ_PSII), the photochemical quenching coefficient (Q_p). However, nonphotochemical quenching coefficient (Q_n) and non-radiative dissipation (NPQ) were decreased. Plants treated with triacontanol at the doses of 0.3 and 3.0 μg had significantly higher yields of fruits than control. No differences were found between plants treated once and twice with the growth regulator. Triacontanol did not show univocal effects on dry matter content in fruits either.     

三十烷醇在原生质体冰冻和化冻中对质膜的保护作用

在合适浓度范围的三十烷醇存在下,经冰冻处理的烟草叶肉原生质体化冻后质膜完整率均高于对照。以0.1,0.5和1.0p,p.m三十烷醇对原生质体质膜的保护效果较佳。三十烷醇对甘蔗叶肉原生质体的抗冰冻力有类似效果,使用浓度亦相近。作者认为三十烷醇可能与质膜结合,在维护质膜正常物相和和生理活性的完整性与稳定性中起一定作用。     

Triacontanol modulates photosynthesis and osmoprotectants in canola (Brassica napus L.) under saline stress

Abstract

An experiment was conducted to assess the effect of pre-sowing seed treatment with triacontanol (TRIA) in canola (Brassica napus L.) cultivar (RBN-3060) under saline stress. Canola seeds were soaked in three levels of TRIA (0, 0.5, and 1 mg L^?1) for 12 hours. Three levels of salt stress (0, 100, and 150 mM NaCl) in full strength Hoagland's nutrient solution were applied to 56-days-old plants. Salt stress caused a significant reduction in growth, gas exchange, photochemical quenching (qP), and shoot and root K⁺ contents, while increased leaf glycine betaine, free proline, and shoot Na⁺ contents. Pre-sowing seed treatment with TRIA increased shoot fresh weight, number of seeds per plant, photosynthetic rate, transpiration rate, ratio of chlorophyll a/b, qP, electron transport rate, shoot and root K⁺ contents, and free proline and glycine betaine contents of canola plants at various TRIA levels under nonsaline or saline conditions.     

Study of interaction effect between triacontanol and nitric oxide on alleviating of oxidative stress arsenic toxicity in coriander seedlings

In this study, triacontanol (TRIA) and nitric oxide (NO) interaction on arsenic (As)-induced oxidative stress tolerance in coriander (Coriandrum sativum L.) plants was investigated. The results showed that As had a significant adverse effect on the plant’s biomass. The seedlings pretreated with TRIA and NO significantly increased growth reduction induced by the metalloid. The obtained results indicated that the application of TRIA and sodium nitroprusside (SNP) generally reduced oxidative markers such as of electrolyte leakage percentage, malondialdehyde and H₂O₂ contents under As toxicity, while application of As treatment without TRIA?+?SNP increased these oxidative parameters compared to the control. The non-enzymatic antioxidant contents such as total phenol, anthocyanin, carotenoid, ascorbic acid and reduced glutathione (GSH) were extracted and assayed from both control and treated plants. It was found that TRIA?+?SNP treatments have a profound effect on the antioxidant metabolism and caused an enhancement in non-enzymatic antioxidant potentials under As toxicity in coriander. Moreover, the results revealed a mutually amplifying reaction between TRIA and NO in reducing As-induced damages.     

The effect of triacontanol on micropropagation of Capsicum frutescens and Decalepis hamiltonii W & A

We studied the effect of triacontanol (TRIA) on shoot multiplication and rooting of in vitro derived shoot tips of Capsicum frutescens and Decalepis hamiltonii W & A. In both shoot multiplication and rooting phases, TRIA was administered at 2-20 g l^–1. TRIA resulted in highest promotion of axillary shoot proliferation at 2 g l^–1 in Capsicum frutescens and 20 g l^–1 in Decalepis hamiltonii while rooting was maximum at 5 and 10 g l^–1 for Capsicum frutescens and Decalepis hamiltonii respectively. TRIA enhanced shoot growth and chlorophyll content of leaves and also influenced root induction and supported growth of the roots. This work reveals that TRIA can be used as an effective growth regulator in the micropropagation of Capsicum frutescens and Decalepis hamiltonii, an endangered shrub of Deccan peninsular India.     

Triacontanol negatively modulates the jasmonic acid-stimulated proteinase inhibitors in tomato (Lycopersicon esculentum)

Triacontanol (TRIA), a long chain aliphatic alcohol (C30H61OH) reverses the effect of jasmonic acid (JA) in inducing proteinase inhibitors (PIs) in tomato leaves. Porcine pancreas trypsin and Spodoptera litura gut proteinases were inhibited in the presence of leaf proteins treated with JA, and TRIA partially reverses this effect. Spodoptera litura larvae fed with tomato leaves treated with JA were reduced in body weight and TRIA is able to partially reverse this JA-induced effect. These results reflect the partial reversal effect of TRIA in down regulating the JA-induced production of proteinase inhibitors.     

苏门答腊金合欢树皮的化学成分研究

从苏门答腊金合欢（Ａｃａｃｉａ ｓｕｍ ａ Ｂｕｃｈ－Ｈａｍ ． ｅｘ Ｗａｌｌ．）树皮干粉的热乙醇提取液中分离到６个化合物,其中４个通过光谱和理化方法鉴定为：正三十烷醇（１）、羽扇豆庚酸酯（２）、β－谷甾醇（３）和胡萝卜甙（５）。化合物２是新化合物,命名为羽扇豆庚酸酯。这些化合物均首次从苏门答腊金合欢中分离得到     

胆固醇和三十烷醇对浮萍开花的影响(英文)

研究了胆固醇和三十烷醇对浮萍开花的影响。两种化合物在不影响营养生长的情况下都能显著地促进短日植物Ｌｅｍｎａａｅｑｕｉｎｏｃｔｉａｌｉｓ６７４６在长日照条件下的开花。胆固醇促进开花的有效浓度范围为１０－８～１０－６ｍｏｌ／Ｌ,三十烷醇促进开花的有效浓度范围为２．３×１０－１４～２．３×１０－８ｍｏｌ／Ｌ。相反,在一定浓度范围内,两种化合物对长日植物Ｌ．ｇｉｂｂａＧ１在长日照条件下的开花则有抑制作用。这些实验结果表明,亲脂化合物能够影响浮萍的开花进程,但是对于长日植物的作用不同于短日植物。