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《Biocatalysis and Biotransformation》2013,31(4):147-150
AbstractGrowing cells of Pseudomonas putida transformed isoeugenol after 5 days of incubation to give mainly vanillin, eugenol, 4-(E)-(3-hydroxyprop-1-enyl)-2-methoxyphenol and the dimeric molecule (+)-4-[2,3-dihydro-7-methoxy-3-methyl-5-(E)-(1-propenyl)-2-benzofuranyl]-2-methoxyphenol (licarin A). The formation of the latter compound from isoeugenol by biotransformation with P. putida is reported here for the first time. 相似文献
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Two kinds of carboxypeptidases (F–I, F–II) were purified from the sarcocarp of watermelon (Citrullus vulgaris, var. Shimao). F–I was not purified to homogeneity. F–II was homogeneous on ultracentrifugal analysis, but a trace of impurity was detected at high concentrations by disc electrophoresis.F–I was optimally active and stable at pH 5.0~5.5 and was strongly inhibited by DFP and HgCl2, but not by EDTA. The molecular weight and isoelectric point were 89,000 and 4.4, respectively.F–II was optimally active at pH 5.0 ~ 5.5 and was most stable at pH 5.5 ~ 7.0. It was completely inhibited by DFP and HgCl2, but not by EDTA and 1, 10-phenanthroline, and it hydrolyzed an oligopeptide containing proline, glutamic acid, lysine and several neutral amino acids, sequentially from the C-terminal. The molecular weight and isolelectric point were 110,000 (5.1 S) and 5.0, respectively.The similarity of enzymatic properties of both the present enzymes to those of other plant carboxypeptidases and pig kidney cathepsin A are discussed. 相似文献
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The investigation of seven Bolivian Senecio species afforded in addition to known compounds five new furanoeremophilanes, two C10-amides, a new isoeugenol ester and the isomer of pterophorin. The structures are elucidated mainly by spectroscopic methods. While most of the new compounds are closely related to those isolated before from Senecio species, four of them are unusual. Another is a new type of a furanoeremophilane; its biogenesis however, can be explained easily from known types. 相似文献
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Different odorants are recognized by different combinations of G protein-coupled olfactory receptors, and thereby, odor identity is determined by a combinatorial receptor code for each odorant. We recently demonstrated that odorants appeared to compete for receptor sites to act as an agonist or an antagonist. Therefore, in natural circumstances where we always perceive a mixture of various odorants, olfactory receptor antagonism between odorants may result in a receptor code for the mixture that cannot be predicted from the codes for its individual components. Here we show that stored isoeugenol has an antagonistic effect on a mouse olfactory receptor, mOR-EG. However, freshly purified isoeugenol did not have an inhibitory effect. Instead, an isoeugenol derivative produced during storage turned out to be a potent competitive antagonist of mOR-EG. Structural analysis revealed that this derivative is an oxidatively dimerized isoeugenol that naturally occurs by oxidative reaction. The current study indicates that as odorants age, they decompose or react with other odorants, which in turn affects responsiveness of an olfactory receptor(s). 相似文献
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Ferdinand Bohlmann Christa Zdero Robert M. King Harold Robinson 《Phytochemistry》1980,19(6):1141-1143
The investigation of Fitchia speciosa afforded, in addition to known compounds, three new sesquiterpene lactones, iso-β-costal, two p-hydroxyacetophenone derivatives and an isoeugenol derivative. The structures were elucidated by spectroscopic methods. The chemotaxonomic situation in this complicated genus is discussed. 相似文献
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Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol 总被引:1,自引:0,他引:1
Dexter R Qualley A Kish CM Ma CJ Koeduka T Nagegowda DA Dudareva N Pichersky E Clark D 《The Plant journal : for cell and molecular biology》2007,49(2):265-275
Petunia flower petals emit large amounts of isoeugenol, which has been shown to be synthesized by isoeugenol synthase (PhIGS1) from an ester of coniferyl alcohol, hypothesized to be coniferyl acetate. This paper describes the identification and characterization of a novel petunia gene encoding an enzyme belonging to the BAHD acyltransferase family whose expression correlates with isoeugenol biosynthesis. RNAi suppression of this gene results in inhibition of isoeugenol biosynthesis. Biochemical characterization of the protein encoded by this gene showed that it has acetyltransferase activity and is most efficient with coniferyl alcohol among the alcohol substrates tested. Overall, these data support the conclusion that coniferyl acetate is the substrate of isoeugenol synthase. 相似文献
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Danny Kessler Celia Diezel David G. Clark Thomas A. Colquhoun Ian T. Baldwin 《Ecology letters》2013,16(3):299-306
Flowers recruit floral visitors for pollination services by emitting fragrances. These scent signals can be intercepted by antagonists such as florivores to locate host plants. Hence, as a consequence of interactions with both mutualists and antagonists, floral bouquets likely consist of both attractive and defensive components. While the attractive functions of floral bouquets have been studied, their defensive function has not, and field‐based evidence for the deterrence of floral‐scent constituents is lacking. In field and glasshouse experiments with five lines of transgenic Petunia x hybrida plants specifically silenced in their ability to release particular components of their floral volatile bouquet, we demonstrate that the emission of single floral‐scent compounds can dramatically decrease damage from generalist florivores. While some compounds are used in host location, others prevent florivory. We conclude that the complex blends that comprise floral scents are likely sculpted by the selective pressures of both pollinators and herbivores. 相似文献
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Antibacterial isoeugenol coating on stainless steel and polyethylene surfaces prevents biofilm growth 下载免费PDF全文
C.K. Nielsen G. Subbiahdoss G. Zeng Z. Salmi J. Kjems T. Mygind T. Snabe R.L. Meyer 《Journal of applied microbiology》2018,124(1):179-187