抑制数个激酶的新药上市改变了药物治疗状况

由于抗癌剂难以奏效，肾细胞癌首选的药物治疗方法还是使用免疫赋活剂。     

油松雌性不育系球果蛋白质双向电泳技术的建立

本文建立了油松雌性不育系雌球果蛋白质组研究中的双向电泳技术.第一向采用固定pH值梯度(IPG)胶条在IPGphorTM等电聚焦仪上进行等电聚焦,第二向在恒功率且恒温条件下于Ettan-DALTTMⅡ高通量电泳仪上进行SDS-PAGE电泳,以银染和考马斯亮蓝两种方法染色.通过对全蛋白的提取、胶条pH值和胶条肿胀等技术环节的优化和比较,得到了重复性很高,分离效果良好的蛋白质双向图谱.     

成花光敏感的雌雄性不育苎麻活性氧代谢研究

为研究成花光敏感雌雄不育苎麻(Floral initiation photo-sensitive male and female sertile ramie,SMSFS)的败育与活性氧代谢的关系,对SMSFS及其回复突变株SMSFS(R)花器官的活性氧(ROS)清除酶活性和丙二醛(MDA)含量测定．结果表明：SMSFS蕾的过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性明显高于可育株SMSFS(R),而膜脂过氧化产物MDA的含量低于SMSFS(R);与蕾期相比,败育期SMSFS雌雄花除POD活性有所上升,而SOD和CAT活性则显著下降,MDA含量升高并高于开花期可育株．可见活性氧代谢的失衡引起了的膜脂过氧化,与SMSFS雌雄性败育明显相关．     

三倍体品种的选育和利用

概述了三倍体生物的两大基本特征(细胞巨大性和不育性)及其产生原因,对有很好经济和社会效益的三倍体杨树、甜菜、无籽西瓜和某些海洋生物选育利用作了简要介绍、展示了三倍体品种选育利用的美好前景。     

胡芦巴水溶性甾体皂苷的提取分离工艺研究

以薯蓣皂苷元的提取率为指标,应用L9(3^4)正交试验设计优化了从脱多糖、脱脂胡芦巴豆粉中提取甾体皂苷的工艺条件,并且筛选了ZTC澄清剂对胡芦巴甾体皂苷水提取液的澄清条件。实验结果表明,影响水提取的主次因素为：提取次数＞提取温度＞固液比＞提取时间;最佳水提取条件为：固液比为1：10,在70℃下提取3次,每次120min得出胡芦巴种子水提取液的澄清条件如下：ZTCl 1澄清剂的加入次序是先加B组分再加入A组分;澄清剂B和A的最佳用量分别为1．0g/L和O．5g／L;加入组分A或B后在80℃下作用30min即可达到澄清目的。     

Influences of insecticides on toxicity and cuticular penetration of abamectin in Helicoverpa armigera

Synergistic actions for mixtures of abamectin with other insecticides in some insect pests were evaluated, and the possible synergistic mechanism was studied by the comparison in toxicity and cuticular penetration of abamectin between with and without other insecticides or synergists in Helicoverpa armigera larvae. The results of bioassay showed that horticultural mineral oil (HMO), hexaflumuron, chlorpyrifos, and some other insecticides were synergistic to abamectin with 152.0-420.0 of co-toxicity coefficient(CTC) in some agricultural insect pests. In topical application tests, HMO or piperonyl butoxide (PBO) increased the toxicity of abamectin in larvae of H. armigera, but the mortality was not affected by s,s,s-tributylphorotrithioate (DEF) and triphenylphosphate (TPP). The synergistic action of HMO was obviously higher than PBO, and when treated simultaneously with abamectin, HMO gave a more significant synergism than if treated 2 hours ahead. The highest synergistic effect (SE) was found in the mixture of ‘abamectin HMO (1:206)‘. The mortality did not increase or the toxicity drop, when a synergist or HMO was added into the mixture of ‘abamectin HMO‘ or ‘abamectin synergist‘, respectively. Results from the isotope tracing experiments showed that HMO significantly enhanced the penetration of ^3H-abamectin through the cuticle of H.armigera larvae, which resulted in the synergism of the mixture. The cuticular penetration of ^3H-abamectin was not accumulatively affected by chlorpyrifos, nor by hexaflumuron,though there was an inhibition within 30 seconds or 1 hour after treated by these two chemicals respectively. Results suggested that the synergism of abamectin mixed with hexaflumuron or chlorpyrifos might be related to inhibition of metabolic enzymes or target sites in the larvae.     

水稻籼粳亚种间杂种低温花粉不育的QTL分析

为探明籼粳杂种低温花粉不育的遗传基础,以籼稻品种3037和粳型广亲和品种02428的F2分离群体进行了低温花粉不育的遗传分析。推迟播种后,F2群体各单株孕穗期的日平均温度为21～23℃,调查了F2群体各单株的花粉育性。利用108对SSR引物构建了包含157个F2单株,覆盖12条染色体的分子标记连锁图谱。该连锁图的总长度为1857．8cM,标记间平均距离为16．26cM,标记较均匀地分布在12条染色体上。采用区间作图法对F2群体花粉不育进行QTL分析,共检测到2个低温花粉不育QTLS,即qLTSPS2和qLTSPS5,分别位于第2、5染色体,其加性效应分别为0．021、0．045,显性效应分别为-0．246、-0．251,显性度分别为11．7和4．8,具有超显性效应．超显性是QTL作用的主要方式,这2个位点杂合基因型在低温环境下具有降低花粉育性的作用,分别解释表型变异的15．6％、11．9％。另外,两因素的方差分析表明这两个QTL之间不存在互作。