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Rolf Bergmann 《Antonie van Leeuwenhoek》1989,55(2):143-152
Thiolutin was found to inhibit the utilization of glucose and other growth substrates in Escherichia coli. The inhibition was detected by a sharp drop of the respiration rate after addition of the antibiotic. The actual function affected was allocated to the cytoplasmic membrane of the bacterial cells by the following evidence:
It was concluded that the transport of certain substrates across the membrane was inhibited. 相似文献
| – | - spheroplasts were affected like intact cells, |
| – | - individual reactions of either the electron transport chain or the glycolytic pathway were not inhibited, |
| – | - glucose consumption in the culture stopped and the cells accumulated guanosine tetraphosphate as under starvation conditions, |
| – | - activation of the cell's apo-glucose dehydrogenase restored respiration via bypassing the glucose phosphotransferase system. |
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