Thiolutin inhibits utilization of glucose and other carbon sources in cells of Escherichia coli |
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Authors: | Rolf Bergmann |
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Institution: | 1. Institut für Allgemeine Botanik, Universit?t Hamburg, Abteilung Mikrobiologie, Ohnhorststra?e 18, D-2000, Hamburg 52, FRG
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Abstract: | Thiolutin was found to inhibit the utilization of glucose and other growth substrates in Escherichia coli. The inhibition was detected by a sharp drop of the respiration rate after addition of the antibiotic. The actual function affected was allocated to the cytoplasmic membrane of the bacterial cells by the following evidence: |
- spheroplasts were affected like intact cells,
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- individual reactions of either the electron transport chain or the glycolytic pathway were not inhibited,
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- glucose consumption in the culture stopped and the cells accumulated guanosine tetraphosphate as under starvation conditions,
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- activation of the cell's apo-glucose dehydrogenase restored respiration via bypassing the glucose phosphotransferase system.
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It was concluded that the transport of certain substrates across the membrane was inhibited. |
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Keywords: | thiolutin Escherichia coli transport respiration |
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