Genetic variation in Armillaria mellea subsp. nipponica estimated using IGS-RFLP and AFLP analyses

In this study, genetic variation of Armillaria mellea subsp. nipponica was estimated using intergenic spacer-restriction fragment length polymorphism (IGS-RFLP) and amplified fragment length polymorphism (AFLP) analyses. Four IGS-RFLP phenotypes were produced, of which two have never been reported. AFLP analysis suggested that the 11 isolates used could be divided into five subgroups, and the isolates within the same subgroup were distributed throughout a relatively large area in Japan. A parental isolate and its offspring (single-spore isolates) showed an almost identical AFLP profile to each other. These results suggest that the large distribution of the isolates within the same subgroup were established via the basidiospore from a common parental strain. Contribution no. 378 of The Tottori Mycological Institute     

同宗配合蜜环菌与欧美异宗配合蜜环菌狭义种的分子系统学关系

从亚洲、欧洲和北美收集18个蜜环菌狭义种菌株,PCR扩增其rDNA的IGS和ITS区域,用AluI、HaeIII、HinfI和TagI四种限制性内切酶进行酶切,同时用随机扩增微卫星(RAMS)多态性,对蜜环菌狭义种的进行了遗传多样性和分子系统学分析。结果蜜环菌狭义种的IGS和ITS-RFLP类型比以前报道的更多,亚洲、欧洲和北美菌株都具有比较明显的特征片段,通过ITS和IGS图谱可将三个大陆的菌株区分开,其中IGS-HinfI图谱能完全区分3个大陆的菌株。RFLP数据的系统学分析表明,该种存在明显的大陆遗传分化,亚、欧、北美的群体分属三个相互独立的进化系,北美群体IGS变异程度较小,ITS变异程度极高;而欧洲群体IGS的变异程度较大,多态性高,ITS变异程度非常小。RAMS系统分析表明,中国、日本和非洲的同宗配合蜜环菌属于一个系统发育系,该发育系同欧洲和北美的异宗配合种的两个发育系分属3个不同的独立进化分支。据此建议欧洲和北美的异宗配合蜜环菌应作为蜜环菌的两个亚种。