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Ya. O. Loginov G. G. Khudaigulov S. P. Chetverikov A. I. Melent’ev O. N. Loginov 《Applied Biochemistry and Microbiology》2011,47(3):311-314
Highly viscous polysaccharide (250–350 kDa) of an alginate nature with a predominance of α-L-guluronic acid (M/G = 0.22) was
obtained from Azotobacter vinelandi. The yield of polysaccharide was 20.5 ± 0.5 g/l when cultured in a medium containing molasses at a viscosity of the cultural
liquid of over 30000 cSt. The biopolymer is stable at pH 4.0–9.0 in a wide temperature range and well soluble in highly mineralized
water; it retains a high viscosity level and can be used in the petroleum industry for enhanced oil recovery. 相似文献
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Application of 1% methol, which, along with cold, activates specific thermosensitive ionic channels, changes the number of functioning cold receptors on the skin of the forearm similarly to the cold exposure test; however, it does not affect the number of heat receptors and does not significantly change the threshold of cold sensation. Group variants of responses to menthol that indicate individual differences in the sensitivity of skin receptors to the effects of methol and cold have been found. The results obtained give grounds to suggest that, from the variant of response to menthol (a decrease, increase, or absence of changes in the number of functioning cold receptors 5 min after menthol application), it is possible to predict specific features of response to cold. 相似文献
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E. P. Isakova Yu. I. Deryabina N. N. Gessler T. A. Belozerskaya Ya. M. Rabinovich 《Applied Biochemistry and Microbiology》2010,46(3):318-323
Cell respiratory activity of protoplasts obtained from the wild type of Neurospora crassa and photoreceptor complex WCC—white collar 1 (wc-1) and white collar 2 (wc-2)—mutants of Neurospora crassa strains was investigated. Respiration inhibition by KCN in the presence of 25 mM succinate was similar in all strains and
did not exceed 83–85% against control. The significant induction of KCN-resistant respiratory pathway occurred under 1% glucose
oxidation in wc-1 and wc-2 mutants if compared with the wild type strains. The inhibitors of the main (cytochrome) pathway of electron transfer in mitochondria—1
mM KCN and antimycin A (4 μg/ml)—blocked the respiration rate of the protoplasts from N. crassa wild type by 75%, while the cell respiration of wc-1 and wc-2 strains was suppressed by approximately 50%. The specific inhibitor of alternative oxidase—10 mM salicylhydroxamic acid (SHAM)—in
combination with the blockers of mitochondrial electron transfer chain caused the total suppression of respiratory activity
of protoplasts in all studied strains. It is supposed that an increase of KCN-resistance in WCC mutants under glucose oxidation
is connected with alternative oxidase activation as the result of failure in reception and signal transduction of active oxygen
species. 相似文献