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I. G. Shirokikh G. M. Zenova O. V. Mersaeva E. V. Lapigina L. V. Lysak 《Biology Bulletin》2006,33(4):404-408
Actinomycete complexes were studied in the rhizosphere of three crop species using luminescence microscopy and plating. The concentration of the total prokaryotic biomass and the length of actinomycete mycelium proved higher in the rhizosphere than in root-free soil. Actinomycetes in the rhizosphere of oats (Avena sativa L.), winter rye (Secale cereale L.), and red clover (Trifolium pratense L.) were represented by the genera Streptomyces and Micromonospora and oligosporous species. The length and biomass of actinomycete mycelium proved to decrease while the generic diversity increased in the following sequence: winter rye—oats—red clover. Increasing soil suppression and plant resistance to phytopathogens using mycelial prokaryotes is discussed in the context of environmental safety. 相似文献
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Shaoyi Sun Richard Dean Qi Jia Alla Zenova Jing Zhong Celene Grayson Clark Xie Andrea Lindgren Pritpaul Samra Luis Sojo Margaret van Heek Linus Lin David Percival Jian-min Fu Michael D. Winther Zaihui Zhang 《Bioorganic & medicinal chemistry》2013,21(24):7724-7734
Endothelial lipase (EL) activity has been implicated in HDL metabolism and in atherosclerotic plaque development; inhibitors are proposed to be efficacious in the treatment of dyslipidemia related cardiovascular disease. We describe here the discovery of a novel class of anthranilic acids EL inhibitors. XEN445 (compound 13) was identified as a potent and selective EL inhibitor, that showed good ADME and PK properties, and demonstrated in vivo efficacy in raising plasma HDLc concentrations in mice. 相似文献
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Investigations showed that micromonosporas, along with streptomycetes, are the major inhabitants of floodplain meadow ecosystems, where their population varies from tens of thousands to hundreds of thousands of CFU per g substrate. In spring, the population of micromonosporas in soil and on the plant roots was found to be denser than that of streptomycetes. 相似文献
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A clear-cut dependence of the distribution of acidophilic actinomycetes on the pH value of soil was established. Acidophilic actinomycetes were found to be present in soils whose pH does not exceed 6.8 (acid forest soils, lowland peaty soil, and ordinary chernozem) and not in slightly alkaline soils (chestnut sodic and alluvial meadow soils). In the acid lowland peaty soil, the species diversity of acidophilic streptomycetes was lesser than the species diversity of streptomycetes revealed in the same soil by using neutral medium. 相似文献
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G. M. Zenova P. A. Kozhevin N. A. Manucharova M. S. Dubrova D. G. Zvyagintsev 《Biology Bulletin》2012,39(5):416-422
It has been demonstrated that complexes of mycelial bacteria (actinomycetes), in which the amount of psychrotolerant actinomycetes reaches hundreds of thousands of CFU/g of the soil (frequently exceeding the portion of mesophilic forms), are developed in peat and podzolic soils of the tundra and taiga at low temperatures. As actinomycetes grow and develop in cold soils, their mycelium increases in length. Use of the molecular in situ hybridization method (fluorescent in situ hybridization, FISH) demonstrated that the portion of metabolically active mycelial actinobacteria exceeds the portion of unicellular actinobacteria in the Actinobacteria phylum. Specific peculiarities of psychrotolerant populations in relation to the spectrum of consumed substrates (histidine, mannitol, saccharose) were established by the method of multirespirometric testing. 相似文献
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N. A. Manucharova A. N. Vlasenko G. M. Zenova T. G. Dobrovol’skaya A. L. Stepanov 《Biology Bulletin》2008,35(5):549-553
A computational method for estimating specific activity of chitin decomposition by microorganisms is proposed. Spectrophotometric and gas chromatographic methods have been used to determine the rates of chitinase production, biomass accumulation, and carbon dioxide emission by pure cultures of microorganisms grown on a chitin-containing medium. Among dominants of the chitinolytic community of chernozem (Trichoderma viride, Stretomyces albolongus, Alcaligenes, and Arthrobacter), the highest chitinolytic activity is characteristic of prokaryotes. In brown desert-steppe soil, the main destructor prokaryotes are actinomycetes (S. roseolilacinus). The biomass of the fungus T. viride growth on the chitin-containing medium markedly exceeds that of prokaryotes, but the specific activity of respiration and chitinase production in actinomycetes S. roseolilacinus and S. albolongus is an order of magnitude higher than in T. viride. 相似文献
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D. G. Zvyagintsev G. M. Zenova E. S. Lobakova G. M. Nikolaev E. O. Omarova E. A. Ivanova I. B. Savelyev 《Microbiology》2010,79(3):314-320
Associations of cyanobacteria and actinomycetes were formed experimentally from the cyanobacterium Anabaena variabilis ATCC 29413 and the streptomycetes isolated from apogeotropic roots of sago plants. Based on their phenotypic properties and
the 16S rRNA gene sequencing, the streptomycetes were identified as representatives of Streptomyces pluricolorescens (strains 1 and 2). Cyanobacteria developing in monoculture and in association with an actinomycete were essentially different
in their morphological and physiological-biochemical characteristics. In associations, cyanobacteria showed a higher (by tens
of times) nitrogen-fixing activity compared to the monoculture and the morphological modifications of which were not observed
in the monoculture (increase in cell size, increase in the portion of heterocysts among vegetative cells, appearance of the
forms of unbalanced growth of cyanobacteria as giant, disc-shaped, curved, and rhomboid cells). At extremely low humidity
(aw 0.50), associated cyanobacterial cells remained viable, whereas in the monoculture, chlorophyll decomposition and cells death
occurred. The methods of high-resolution (H1 600 MHz) nuclear magnetic resonance (NMR) and pulsed-gradient spin echo NMR revealed a fraction of mobile protons in lyophilized
samples of the cyanobacterium-actinomycete association, which was evidence of the presence of free water. This fraction was
not found in the lyophilized samples of cyanobacterial and streptomycete monocultures. The revealed differences can explain
the survival of cyanobacterial cells in associations. 相似文献
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G M Zenova 《Mikrobiologiia》1968,37(3):454-459