全文获取类型
收费全文 | 748篇 |
免费 | 30篇 |
出版年
2021年 | 7篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 12篇 |
2015年 | 8篇 |
2014年 | 16篇 |
2013年 | 55篇 |
2012年 | 47篇 |
2011年 | 40篇 |
2010年 | 23篇 |
2009年 | 25篇 |
2008年 | 31篇 |
2007年 | 26篇 |
2006年 | 33篇 |
2005年 | 45篇 |
2004年 | 41篇 |
2003年 | 45篇 |
2002年 | 40篇 |
2001年 | 15篇 |
2000年 | 8篇 |
1999年 | 12篇 |
1998年 | 9篇 |
1997年 | 5篇 |
1996年 | 12篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 9篇 |
1992年 | 13篇 |
1991年 | 11篇 |
1990年 | 12篇 |
1989年 | 13篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 8篇 |
1985年 | 17篇 |
1984年 | 13篇 |
1983年 | 6篇 |
1982年 | 8篇 |
1981年 | 16篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 7篇 |
1976年 | 9篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 3篇 |
1970年 | 2篇 |
1967年 | 1篇 |
排序方式: 共有778条查询结果,搜索用时 15 毫秒
1.
2.
Sumio Tanase Yoshimasa Morino 《Biochemical and biophysical research communications》1976,68(4):1301-1308
L-Propargylglycine serves as an amino acid substrate in the transamination reaction catalyzed by both cytosolic and mitochondrial aspartate aminotransferases from pig heart. Incubation of these isoenzymes with L-propargylglycine alone did not result in the inactivation of these enzymes. However, the presence of 2-oxoglutarate or pyruvate caused gradual irreversible inactivation of these isoenzymes. The inactivation was greatly accelerated by the presence of formate ion. Inactivation of both isoenzymes with L-[2-14C]propargylglycine resulted in stoichiometric incorporation of the radioactive molecule. Drastic changes in the absorption and circular dichroic spectra of the enzymes which took place during the inactivation also indicated that the modification by L-propargylglycine is restricted to the active site of these isoenzymes. 相似文献
3.
Dr. Kimie Fukuyama Yoshimasa Ito Kazuo Yabe William L. Epstein 《Cell and tissue research》1985,240(2):417-423
Summary Monospecific antibody directed to cysteine protease of 2-day-old rat epidermis recently characterized as being different from the proteases previously reported was produced in rabbits. By immunofluorescence microscopy and immunoperoxidase staining with an avidin-biotin-peroxidase method the protease was found to be present in the epidermis of rodents of different ages as well as that of humans, but not in the dermis. The staining in germinative cells was more intense than in cells in the superficial layers. It appeared as irregular patches in the nuclei and stained more diffusely in the cytoplasm where small granular components, strongly stained, were identified. The staining patterns in granular cells showed accumulation of the antigen in a granular form. The morphology and distribution of granules resembled those of keratohyalin-like granules in the nucleus and dense homogenous deposits in the cytoplasm. In cornified cells the reaction product was localized by the plasma membrane where concentration of the dense homogenous deposits occurred, suggesting that the cysteine protease is one component of the unique and characteristic structure of differentiated keratinocytes. In addition, the cysteine protease antigen having the same molecular weight as the epidermal enzyme was detected in liver, kidney and lung indicating a wider tissue distribution of the protease. The significance of the protease in regulation of cellular functions remains to be investigated. 相似文献
4.
5.
6.
Takaki Hiwasa Jun Ma Yoshimasa Ike Nobuhiko Katunuma Shigeru Sakiyama 《Cell biochemistry and function》1995,13(4):293-296
Degradation of cyclin B was effectively suppressed when cells were treated with ALLN (N-acetylleucylleucylnorleucinal) which inhibits proteasome, calpain and cysteine proteinase cathepsins. In order to examine which protease degrades cyclin B, the effect of a cathepsin inhibitor, cystatin α, was investigated. The cystatin α gene was inserted into an inducible expression vector, pMSG, and transfected into NIH3T3 mouse fibroblasts. The expression of cystatin α was induced effectively in the transfected cells after treatment with dexamethasone. Overexpression of cystatin α resulted in an increase of the amount of cyclin B, suggesting that cysteine proteinase cathepsins might be involved in the degradation of cyclin B. 相似文献
7.
8.
9.
The verification of a number of assumptions made about cycloamylose structure, substrate binding and catalysis is dependent on the ability to 3-O-alkylate selectively these oligosaccharides. In the presence of barium oxide and barium hydroxide octahydrate, cycloheptaamylose reacts with 3-bromopropene in dimethylformamide and dimethyl sulfoxide to produce a high yield of tetradecakis-2,6-O-allylcycloheptaamylose, a compound which can serve as a highly versatile intermediate in the preparation of heptakis-3-O-alkylated cycloheptamyloses. Depending on the nature of the substituent to be introduced at the C-3 hydroxyl position, the intermediate allyl ether can be 3-O-alkylated and the allyl ether can be isomerized to the vinyl ether and cleaved; or, alternatively, the allyl ether can be first subjected to isomerization followed by alkylation, and then cleavage. 相似文献
10.
Ana C.A. Casarotti Daniela Teixeira Ieda M. Longo-Maugeri Mayari E. Ishimura Maria E.R. Coste Henrique T. Bianco Flavio T. Moreira Amanda F. Bacchin Maria C. Izar Iran Gonalves Jr Adriano Caixeta Gilberto Szarf Ibraim M. Pinto Francisco A. Fonseca 《Bioscience reports》2021,41(2)
Despite early reperfusion, patients with ST segment elevation myocardial infarction (STEMI) may present large myocardial necrosis and significant impairment of ventricular function. The present study aimed to evaluate the role of subtypes of B lymphocytes and related cytokines in the infarcted mass and left ventricular ejection fraction obtained by cardiac magnetic resonance imaging performed after 30 days of STEMI. This prospective study included 120 subjects with STEMI submitted to pharmacoinvasive strategy. Blood samples were collected in subjects in the first (D1) and 30th (D30) days post STEMI. The amount of CD11b+ B1 lymphocytes (cells/ml) at D1 were related to the infarcted mass (rho = 0.43; P=0.033), measured by cardiac MRI at D30. These B1 cells were associated with CD4+ T lymphocytes at D1 and D30, while B2 classic lymphocytes at day 30 were related to left ventricular ejection fraction (LVEF). Higher titers of circulating IL-4 and IL-10 were observed at D30 versus D1 (P=0.013 and P<0.001, respectively). Titers of IL-6 at D1 were associated with infarcted mass (rho = 0.41, P<0.001) and inversely related to LVEF (rho = −0.38, P<0.001). After multiple linear regression analysis, high-sensitivity troponin T and IL-6 collected at day 1 were independent predictors of infarcted mass and, at day 30, only HDL-C. Regarding LVEF, high-sensitivity troponin T and high-sensitivity C-reactive protein were independent predictors at day 1, and B2 classic lymphocytes, at day 30. In subjects with STEMI, despite early reperfusion, the amount of infarcted mass and ventricular performance were related to inflammatory responses triggered by circulating B lymphocytes. 相似文献