全文获取类型
收费全文 | 876篇 |
免费 | 61篇 |
国内免费 | 1篇 |
出版年
2021年 | 9篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 14篇 |
2015年 | 21篇 |
2014年 | 24篇 |
2013年 | 36篇 |
2012年 | 50篇 |
2011年 | 55篇 |
2010年 | 29篇 |
2009年 | 25篇 |
2008年 | 48篇 |
2007年 | 44篇 |
2006年 | 53篇 |
2005年 | 34篇 |
2004年 | 48篇 |
2003年 | 50篇 |
2002年 | 39篇 |
2001年 | 23篇 |
2000年 | 24篇 |
1999年 | 20篇 |
1998年 | 20篇 |
1997年 | 14篇 |
1996年 | 8篇 |
1995年 | 10篇 |
1994年 | 6篇 |
1993年 | 17篇 |
1992年 | 11篇 |
1991年 | 5篇 |
1990年 | 11篇 |
1989年 | 14篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 15篇 |
1985年 | 12篇 |
1984年 | 13篇 |
1983年 | 6篇 |
1982年 | 8篇 |
1981年 | 8篇 |
1980年 | 12篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1975年 | 7篇 |
1974年 | 4篇 |
1973年 | 6篇 |
1972年 | 7篇 |
1971年 | 3篇 |
1969年 | 6篇 |
排序方式: 共有938条查询结果,搜索用时 15 毫秒
1.
In the albino mutant of an Okinawa strain of Locusta migratoria (L.) (Orthoptera: Acrididae), albinism is caused by the absence of the dark‐colour‐inducing neurohormone (DCIN), which is present in the corpora cardiaca (CC) of normally coloured phenotypes. This study tests whether the absence of DCIN is responsible for albinism in an albino mutant of another locust, Schistocerca gregaria (Forsk.) (Orthoptera: Acrididae). This seemed feasible because a single Mendelian unit controls albinism in both species. However, implantation of CC, or injection of an extract of CC, from albino donors of S. gregaria, induce dark coloration in crowded nymph recipients of the Okinawa albino mutant of L. migratoria, as effectively as do implanted CC, or injections of extract of CC, from normal phenotype donors of S. gregaria. Therefore, DCIN is present in the albino mutant of S. gregaria, and consequently, the albinism in this mutant is not caused by its absence. Implantation of CC, or injection of extracts of CC, from albino donors of S. gregaria to conspecific albino nymphs does not induce darkening. Only extremely high doses of synthetic DCIN injected into albino nymphs of S. gregaria are effective, inducing some darkening. The dose to induce such darkening in albino nymphs of S. gregaria is 50 nmol, ≈ 5 × 106 times higher than that (10 femtomol) needed to induce equivalent darkening in nymphs of the Okinawa albinos of L. migratoria. The results are discussed and some possible explanations of the observed effects outlined. 相似文献
2.
3.
The effects of a nonionic detergent and also of crude oil-detergent mixtures in aqueous solutions were tested by allozyme frequencies at six loci in the Mediterranean marine gastropod Cerithium scabridum Philippi 1849. Our results indicated differential survivorship of allozyme genotypes for both detergent alone and for crude oil-detergent mixtures. This statement is true for all the loci involved in the study. When analyzing each locus separately, two of the six loci (phosphoglucose isomerase and alkaline phosphatase) did not show differential survivorship in detergent pollution. But analyzing the genetic complex involving these two loci we found differential survivorship of specific genotypes.These results reflect the adaptive nature of some PGI genotypes in these marine gastropods and seem inconsistent with the neutral theory of allozyme polymorphisms. Furthermore, these findings suggest that allozyme variants, either singly or in a two-locus structure, demonstrate a differential tolerance to these organic pollutants and can, therefore, be used as detectors of organic pollutants in the sea, as we earlier showed for single loci in two other species of marine gastropods, genus Monodonta (Lavie et al., Environ. Res. 35: 270–276 (1984). 相似文献
4.
Carl W. White Dee-dee H. Nguyen Keiichiro Suzuki Naoyuki Taniguchi Lee S. Rusakow Karen B. Avraham Yoram Groner 《Free radical biology & medicine》1993,15(6):629-636
In evaluating the relative expression of copper-zinc and manganese superoxide dismutase (CuZnSOD and MnSOD) in vivo in states like Down syndrome in which one dismutase is present at increased levels, we measured activities of both enzymes, in tissues of control and transgenic mice constitutively expressing increased levels of CuZnSOD, during exposure to normal and elevated oxygen tensions. Using SOD gel electrophoresis assay, CuZnSOD and MnSOD activities of brain, lung, heart, kidney, and liver from mice exposed to either normal (21%) or elevated (>99% oxygen, 630 torr) oxygen tensions for 120 h were compared. Whereas CuZnSOD activity was elevated in tissues of transgenic relative to control mice under both normoxic or hyperoxic conditions, MnSOD activities in organs of transgenic mice were remarkably similar to those of controls under both conditions. To confirm the accuracy of this method in quantitating MnSOD relative to CuZnSOD expression, two other methods were utilized. In lung, which is the organ exposed to the highest oxygen tension during ambient hyperoxia, a sensitive, specific ELISA for MnSOD was used. Again, MnSOD protein was not different in transgenic relative to control mice during exposure to air or hyperoxia. In addition, lung MnSOD protein was not changed significantly by exposure to hyperoxia in either group. In kidney, a mitochondrion-rich organ, SOD assay, before and after inactivation of CuZnSOD with diethyldithiocarbamate, was used. MnSOD activity was not different in organs from air-exposed transgenic relative to control mice. The data indicated that expression of MnSOD in vivo was not affected by overexpression of the CuZnSOD and, therefore, the two enzymes are probably regulated independently. 相似文献
5.
6.
7.
Abraham Amsterdam Avinoam Reches Yehudith Amir Yael Mintz Yoram Salomon 《Biochimica et Biophysica Acta (BBA)/General Subjects》1978,544(2):273-283
Heparin was found to be the most potent inhibitor of rat ovarian luteinizing hormone-sensitive adenylate cyclase (I50 = 2 μg/ml) when compared to other naturally occurring glycosaminoglycans. This inhinibition was also appparent when this enzyme was stimulated by follicle-stimulating hormone or prostaglandin E 2. Heparin was also found to inhibit glucagon-sensitive rat hepatice adenylate cyclase, and the prostaglandin E1-sensitive enzyme from rat ileum and human platelets. In contrast, heparin stimulated the dopamine sensitive adenylate cyclase from rat caudate nucleus. The sulfade polysugar dextran sulfate exerts similar effects on adenylate cyclase activity of the rat ovary was shown to inhibit hormone binding to rat ovarian plasma membrane in a manner similar to that exerted by heparin. In contrast to heparin, dextran sulfate inhibited dopamine-sensitive adenylate cyclase from rat caudate nucleus. 相似文献
8.
Ruben Attali Sharon Aharoni Susan Treves Ori Rokach Michal Becker Cohen Yakov Fellig Rachel Straussberg Talya Dor Muhannad Daana Stella Mitrani-Rosenbaum Yoram Nevo 《PloS one》2013,8(7)
We describe an autosomal recessive heterogeneous congenital myopathy in a large consanguineous family. The disease is characterized by variable severity, progressive course in 3 of 4 patients, myopathic face without ophthalmoplegia and proximal muscle weakness. Absence of cores was noted in all patients. Genome wide linkage analysis revealed a single locus on chromosome 19q13 with Zmax = 3.86 at θ = 0.0 and homozygosity of the polymorphic markers at this locus in patients. Direct sequencing of the main candidate gene within the candidate region, RYR1, was performed. A novel homozygous A to G nucleotide substitution (p.Y3016C) within exon 60 of the RYR1 gene was found in patients. ARMS PCR was used to screen for the mutation in all available family members and in an additional 150 healthy individuals. This procedure confirmed sequence analysis and did not reveal the A to G mutation (p.Y3016C) in 300 chromosomes from healthy individuals. Functional analysis on EBV immortalized cell lines showed no effect of the mutation on RyR1 pharmacological activation or the content of intracellular Ca2+ stores. Western blot analysis demonstrated a significant reduction of the RyR1 protein in the patient’s muscle concomitant with a reduction of the DHPRα1.1 protein. This novel mutation resulting in RyR1 protein decrease causes heterogeneous clinical presentation, including slow progression course and absence of centrally localized cores on muscle biopsy. We suggest that RYR1 related myopathy should be considered in a wide variety of clinical and pathological presentation in childhood myopathies. 相似文献
9.
Yael Klin Alexander Zlotnik Matthew Boyko Yoram Shapira 《Biochemical and biophysical research communications》2010,399(4):694-698
Excess l-glutamate (glutamate) levels in brain interstitial and cerebrospinal fluids (ISF and CSF, respectively) are the hallmark of several neurodegenerative conditions such as stroke, traumatic brain injury or amyotrophic lateral sclerosis. Its removal could prevent the glutamate excitotoxicity that causes long-lasting neurological deficits. As in previous studies, we have established the role of blood glutamate levels in brain neuroprotection, we have now investigated the contribution of the peripheral organs to the homeostasis of glutamate in blood. We have administered naive rats with intravenous injections of either l-[1-14C] Glutamic acid (l-[1-14C] Glu), l-[G-3H] Glutamic acid (l-[G-3H] Glu) or d-[2,3-3H] Aspartic acid (d-[2,3-3H] Asp), a non-metabolized analog of glutamate, and have followed their distribution into peripheral organs. We have observed that the decay of the radioactivity associated with l-[1-14C] Glu and l-[G-3H] Glu was faster than that associated with glutamate non-metabolized analog, d-[2,3-3H] Asp. l-[1-14C] Glu was subjected in blood to a rapid decarboxylation with the loss of 14CO2. The three major sequestrating organs, serving as depots for the eliminated glutamate and/or its metabolites were skeletal muscle, liver and gut, contributing together 92% or 87% of total l-[U-14C] Glu or d-[2,3-3H] Asp radioactivity capture. l-[U-14C] Glu and d-[2,3-3H] Asp showed a different organ sequestration pattern. We conclude that glutamate is rapidly eliminated from the blood into peripheral tissues, mainly in non-metabolized form. The liver plays a central role in glutamate metabolism and serves as an origin for glutamate metabolites that redistribute into skeletal muscle and gut. The findings of this study suggest now that pharmacological manipulations that reduce the liver glutamate release rate or cause a boosting of the skeletal muscle glutamate pumping rate are likely to cause brain neuroprotection. 相似文献
10.
Rinat Friedman Ayelet Haimy Yoram Epstein 《Computer methods in biomechanics and biomedical engineering》2019,22(3):229-242
Despite the progress in developing personal combat-protective gear, eye and brain injuries are still widely common and carry fatal or long-term repercussions. The complex nature of the cranial tissues suggests that simple methods (e.g. crash-dummies) for testing the effectiveness of personal protective gear against non-penetrating impacts are both expensive and ineffective, and there are ethical issues in using animal or cadavers. The present work presents a versatile testing framework for quantitatively evaluating protective performances of head and eye combat-protective gear, against non-penetrating impacts. The biomimetic finite element (FE) head model that was developed provides realistic representation of cranial structure and tissue properties. Simulated crash impact results were validated against a former cadaveric study and by using a crash-phantom developed in our lab. The model was then fitted with various helmet and goggle designs onto which a non-penetrating ballistic impact was applied. Example data show that reduction of the elastic and shear moduli by 30% and 80% respectively of the helmet outer Kevlar-29 layer, lowered intracranial pressures by 20%. Our modeling suggests that the level of stresses that develop in brain tissues, which ultimately cause the brain damage, cannot be predicted solely by the properties of the helmet/goggle materials. We further found that a reduced contact area between goggles and face is a key factor in reducing the mechanical loads transmitted to the optic nerve and eye balls following an impact. Overall, this work demonstrates the simplicity, flexibility and usefulness for development, evaluation, and testing of combat-protective equipment using computational modeling.
- Highlights
A finite element head model was developed for testing head gear.
Reduced helmet’s outer layer elastic and shear moduli lowered intracranial stresses.
Gear material properties could not fully predict impact-related stress in the brain.
Reduced goggles-face contact lowered transmitted loads to the optic nerve and eyes.