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1.
Oryzopsis contracta is found only in the Big Hollow area of southern Wyoming where it exists sympatrically with the widely distributed species O. hymenoides and O. micrantha. The three species are probably reproductively isolated from each other. Oryzopsis contracta is very similar to O. hymenoides; however, they can be separated on several characters. It also resembles O. micrantha in several characters. This suggests that O. contracta may have evolved through hybridization between the other two species. A quantitative analysis shows that O. contracta occupies an intermediate and central position between the other two species with regard to most characters analyzed. Scatter diagrams reveal a correlated deviation of O. contracta individuals from O. hymenoides toward O. micrantha with respect to several characters. Meiosis in occasional sterile O. hymenoides × O. contracta hybrids shows multivalent associations, suggesting that an appreciable part of the presumed micrantha contribution to the contracta genome was in the form of relatively large chromosome segments. The data suggest that O. contracta evolved as a result of chromosomal translocations in a chance hybrid between O. hymenoides and O. micrantha followed by backcrossing to O. hymenoides and subsequent self-fertilization of the hybrid. 相似文献
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Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging 下载免费PDF全文
Assaf Ben‐Meir Eliezer Burstein Aluet Borrego‐Alvarez Jasmine Chong Ellen Wong Tetyana Yavorska Taline Naranian Maggie Chi Ying Wang Yaakov Bentov Jennifer Alexis James Meriano Hoon‐Ki Sung David L. Gasser Kelle H. Moley Siegfried Hekimi Robert F. Casper Andrea Jurisicova 《Aging cell》2015,14(5):887-895
Female reproductive capacity declines dramatically in the fourth decade of life as a result of an age‐related decrease in oocyte quality and quantity. The primary causes of reproductive aging and the molecular factors responsible for decreased oocyte quality remain elusive. Here, we show that aging of the female germ line is accompanied by mitochondrial dysfunction associated with decreased oxidative phosphorylation and reduced Adenosine tri‐phosphate (ATP) level. Diminished expression of the enzymes responsible for CoQ production, Pdss2 and Coq6, was observed in oocytes of older females in both mouse and human. The age‐related decline in oocyte quality and quantity could be reversed by the administration of CoQ10. Oocyte‐specific disruption of Pdss2 recapitulated many of the mitochondrial and reproductive phenotypes observed in the old females including reduced ATP production and increased meiotic spindle abnormalities, resulting in infertility. Ovarian reserve in the oocyte‐specific Pdss2‐deficient animals was diminished, leading to premature ovarian failure which could be prevented by maternal dietary administration of CoQ10. We conclude that impaired mitochondrial performance created by suboptimal CoQ10 availability can drive age‐associated oocyte deficits causing infertility. 相似文献
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Yu Zhang Yair Eliav Seung-uon Shin Taylor H. Schreiber Eckhard R. Podack Tamar Tadmor Joseph D. Rosenblatt 《Cancer immunology, immunotherapy : CII》2013,62(1):87-99
The mechanisms by which B lymphocytes inhibit anti-tumor immunity remain poorly understood. Murine EMT-6 mammary tumors grow readily in immune competent mice (BALB/c), but poorly in B-cell-deficient μ?/? BALB/c mice (BCDM). T regulatory cell (Treg) expansion and function were impaired in BCDM compared with BALB/c. In this study, we compared tumor growth, Treg cell proliferation, tumor lymphocyte infiltration and cytolytic T cell activity in BALB/c, BCDM and BCDM partially reconstituted with B cells by adoptive transfer (BCDM+B). Partial reconstitution of BCDM with adoptively transferred B cells restored EMT-6 tumor growth, which was independent of IL-10 secretion by B cells. Instead, high frequencies of intratumoral B cells were associated with increased recruitment and proliferation of Treg cells within the tumor microenvironment. The B-cell-dependent accumulation of Treg within the tumor microenvironment was associated with reduced tumor infiltration by CD49+ NK and CD8+ T cells and reduced cytotoxic T cell activity against EMT-6 targets. Our studies indicate that tumor-dependent immunosuppression of T-cell-mediated anti-tumor immunity is coordinated within the tumor microenvironment by B-cell-dependent cross talk with Treg cells, which does not require production of IL-10 by B cells. 相似文献
7.
Shalom W. Applebaum Uriel Tadmor Haggai Podoler 《Entomologia Experimentalis et Applicata》1970,13(1):61-70
A heteropolysaccharide fraction was isolated from Phaseolus vulgaris beans in which it comprises at least 1% of the dry weight of the beans. This heteropolysaccharide increases larval mortality and decreases the rate of larval development and the number of eggs deposited by females of Callosobruchus chinensis, when incorporated in artificial beans in which the larvae feed. It is composed of galactose, glucose, xylose, arabinose and traces of rhamnose, as determined after acid hydrolysis. Of these individual sugars, arabinose and xylose affect adult fecundity as well. However, partial enzymic hydrolysis of the heteropolysaccharide fraction by C. chinensis larval midgut contents releases only glucose, galactose and trace amounts of arabinose, and the integral structure of heteropolysaccharide may be necessary for biological activity. the incorporation of the starch granules of Phaseolus vulgaris beans into artificial beans increases larval mortality and decreases the rate of larval development of C. chinensis. It is suggested that the heteropolysaccharide fraction as well as the starch are part of a complex of natural components of Phaseolus vulgaris beans that make these beans resistant to C. chinensis.
Supported in part by the Research and Development Authority of the Hebrew University of Jerusalem. 相似文献
Résumé Ce travail cherche à préciser pourquoi les graines de haricot (Phaseolus vulgaris) ne sont pas attaquées par la Buche chinoise (Callosobruchus chinensis), espèce cependant très polyphage.Les graines de haricot renferment 1% d'un hétéropolysaccharide, qui s'est révélé accroître la mortalité et ralentir le développement des larves de cette Bruche, mais qui aussi réduit la fécondité des femelles adultes. Ces observations ont été faites à partir d'élevages sur des milieux artificiels normalement favorables, présentés sous forme de haricots, par un moulage approprié.Une hydrolyse acide de cet hétéropolysaccharide libère du galactose, du glucose, du xylose, de l'arabinose et des traces de rhamnose. Ces sucres simples sont testés: l'arabinose et le rhamnose influencent le développement des larves, tandis que le galactose, le xylose et à nouveau l'arabinose, ont un effet sur la fécondité des femelles (réduction de la fécondité à 40% de la normale avec 1% d'arabinose).Toutefois l'hydrolyse enzymatique de cette fraction hétéropolysaccharidique, par le contenu stomacal de larves de C. chinensis reste partielle et libère seulement du glucose, du galactose et des traces d'arabinose. II est possible que la structure intégrale de l'hétéropolysaccharide soit nécessaire à son activité biologique.Un autre facteur défavorable à C. chinensis pourrait être la nature même des grains d'amidon de Phaseolus. Cet amidon incorporé à l'aliment artificiel accroît en effet la mortalité larvaire et ralentit la vitesse de développement. Cet effet défavorable pourrait être dû à la non digestibilité des grains d'amidon entiers.Il est suggéré que la fraction hétéropolysaccharide et l'amidon de Phaseolus sont deux des facteurs présents dans la graine de haricot qui lui confèrent sa résistance naturelle à la Bruche chinoise.
Supported in part by the Research and Development Authority of the Hebrew University of Jerusalem. 相似文献
8.
Referee: Dr. T.J. Higgins, Chief Research Scientist, CSIRO, Divistion of Plant Industry, Clunies Ross Street, Box 1600, Canberra, 2601, Australia Recent advances in gene isolation, plant transformation, and genetic engineering are being used extensively to alter metabolic pathways in plants by tailormade modifications to single or multiple genes. Many of these modifications are directed toward increasing the nutritional value of plant-derived foods and feeds. These approaches are based on rapidly growing basic knowledge, understanding, and predictions of metabolic fluxes and networks. Some of the predictions appear to be accurate, while others are not, reflecting the fact that plant metabolism is more complex than we presently understand. Tailor-made modifications of plant metabolism has so far been directed into improving the levels of primary metabolites that are essential for growth and development of humans and their livestock. Yet, the list of improved metabolites is expected to grow tremendously after new discoveries in nutritional, medical, and health sciences. Despite our extensive knowledge of metabolic networks, many of the genes encoding enzymes, particularly those involved in secondary metabolism, are still unknown. These genes are being discovered at an accelerated rate by recent advances in genetic and genomics approaches. In the present review, we discuss examples in which the nutritional and health values of plant-derived foods and feeds were improved by metabolic engineering. These include modifications of the levels of several essential amino acids, lipids, fatty acids, minerals, nutraceuticals, antinutritional compounds, and aromas. 相似文献
9.
Enhanced Levels of the Aroma and Flavor Compound S-Linalool by Metabolic Engineering of the Terpenoid Pathway in Tomato Fruits 总被引:20,自引:0,他引:20
Efraim Lewinsohn Fernond Schalechet Jack Wilkinson Kenji Matsui Yaakov Tadmor Kyoung-Hee Nam Orit Amar Elena Lastochkin Olga Larkov Uzi Ravid William Hiatt Shimon Gepstein Eran Pichersky 《Plant physiology》2001,127(3):1256-1265
The aromas of fruits, vegetables, and flowers are mixtures of volatile metabolites, often present in parts per billion levels or less. We show here that tomato (Lycopersicon esculentum Mill.) plants transgenic for a heterologous Clarkia breweri S-linalool synthase (LIS) gene, under the control of the tomato late-ripening-specific E8 promoter, synthesize and accumulate S-linalool and 8-hydroxylinalool in ripening fruits. Apart from the difference in volatiles, no other phenotypic alterations were noted, including the levels of other terpenoids such as gamma- and alpha-tocopherols, lycopene, beta-carotene, and lutein. Our studies indicate that it is possible to enhance the levels of monoterpenes in ripening fruits by metabolic engineering. 相似文献