全文获取类型
收费全文 | 18408篇 |
免费 | 2099篇 |
国内免费 | 1813篇 |
出版年
2024年 | 16篇 |
2023年 | 233篇 |
2022年 | 326篇 |
2021年 | 922篇 |
2020年 | 745篇 |
2019年 | 967篇 |
2018年 | 925篇 |
2017年 | 708篇 |
2016年 | 905篇 |
2015年 | 1181篇 |
2014年 | 1399篇 |
2013年 | 1424篇 |
2012年 | 1660篇 |
2011年 | 1503篇 |
2010年 | 936篇 |
2009年 | 880篇 |
2008年 | 956篇 |
2007年 | 840篇 |
2006年 | 680篇 |
2005年 | 613篇 |
2004年 | 637篇 |
2003年 | 675篇 |
2002年 | 652篇 |
2001年 | 501篇 |
2000年 | 394篇 |
1999年 | 320篇 |
1998年 | 229篇 |
1997年 | 163篇 |
1996年 | 149篇 |
1995年 | 107篇 |
1994年 | 99篇 |
1993年 | 77篇 |
1992年 | 73篇 |
1991年 | 90篇 |
1990年 | 72篇 |
1989年 | 57篇 |
1988年 | 37篇 |
1987年 | 38篇 |
1986年 | 30篇 |
1985年 | 32篇 |
1984年 | 14篇 |
1983年 | 11篇 |
1982年 | 10篇 |
1981年 | 7篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1973年 | 4篇 |
1966年 | 2篇 |
1948年 | 1篇 |
1947年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
Janne Alahuhta Sarian Kosten Munemitsu Akasaka Dominique Auderset Mattia M. Azzella Rossano Bolpagni Claudia P. Bove Patricia A. Chambers Eglantine Chappuis John Clayton Mary de Winton Frauke Ecke Esperança Gacia Gana Gecheva Patrick Grillas Jennifer Hauxwell Seppo Hellsten Jan Hjort Mark V. Hoyer Christiane Ilg Agnieszka Kolada Minna Kuoppala Torben Lauridsen En Hua Li Balázs A. Lukács Marit Mjelde Alison Mikulyuk Roger P. Mormul Jun Nishihiro Beat Oertli Laila Rhazi Mouhssine Rhazi Laura Sass Christine Schranz Martin Søndergaard Takashi Yamanouchi Qing Yu Haijun Wang Nigel Willby Xiao Ke Zhang Jani Heino 《Journal of Biogeography》2017,44(8):1758-1769
4.
5.
6.
Jiefeng He Haichao Zhao Dongfeng Deng Yadong Wang Xiao Zhang Haoliang Zhao Zongquan Xu 《Journal of cellular physiology》2020,235(3):2464-2477
This study aimed to identify significant biomarkers related to the prognosis of liver cancer using long noncoding RNA (lncRNA)-associated competing endogenous RNAs (ceRNAs) analysis. Differentially expressed mRNA and lncRNAs between liver cancer and paracancerous tissues were screened, and the functions of these mRNAs were predicted by gene ontology and pathway enrichment analyses. A ceRNA network consisting of differentially expressed mRNAs and lncRNAs was constructed. LncRNA FENDRR and lncRNA HAND2-AS1 were hub nodes in the ceRNA network. A risk score assessment model consisting of eight genes (PDE2A, ESR1, FBLN5, ALDH8A1, AKR1D1, EHHADH, ADRA1A, and GNE) associated with prognosis were developed. Multivariate Cox regression suggested that both pathologic_T and risk group could be regarded as independent prognostic factors. Furthermore, a nomogram model consisting of pathologic_T and risk group showed a good prediction ability for predicting the survival rate of liver cancer patients. The nomogram model consisting of pathologic_T and a risk score assessment model could be regarded as an independent factor for predicting prognosis of liver cancer. 相似文献
7.
The adoptive transfer of sensitized lymphocytes is an effective means to mediate the regression of established tumors. However, successful therapy can only be demonstrated in animal models where tumors are intrinsically immunogenic, capable of eliciting systemic immunity. To explore the potential of this therapeutic approach to tumors of less immunogenicity, we have selected and used a murine tumor, MCA 102, for the current study because all attempts to immunize syngeneic mice failed. We report here that inoculation of mice with a mixture of tumor cells and a bacterial adjuvant, Corynebacterium parvum led to the production of sensitized, but not fully functional, lymphocytes in the draining lymph nodes (LN). These cells, termed pre-effector cells, could nevertheless further differentiate to acquire full immunologic function by an established in vitro sensitization culture method. In adoptive immunotherapy experiments, transfer of as few as 1.5 X 10(7) in vitro sensitized cells not only reduced established pulmonary MCA 102 metastases but also prolonged survival and cured tumors in a majority of the treated animals. In order to elicit pre-effector cells in vivo, inoculation with both tumor cells and C. parvum was essential. Although a broad range of numbers of MCA 102 tumor cells appeared to be effective, generation of pre-effector cells was dependent on the dose of C. parvum. We have found that a C. parvum dose of 25 micrograms was optimal, whereas higher doses of the adjuvant had suppressive effects. Analysis of the kinetics of their appearance revealed that the generation of pre-effector cells was transient. They were detectable 7 days after in vivo priming followed by a rapid decline. Furthermore, pre-effector cells were detected only in the regional draining LN. No reactivity was demonstrable in the spleen, mesenteric LN, PBL, or bone marrow. Taken together, these results expand the scope of immunotherapy by demonstrating the feasibility of manipulating a limited and obscure immune response to the MCA 102 tumor for therapeutic efficacy. 相似文献
8.
Research has revealed that most chlorophyllous explants/plants in vitro have the ability to grow photoautotrophically (without
sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to poor photosynthetic
ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Moreover, numerous studies have been conducted on improving
the in vitro environment and investigating its effects on growth and development of cultures/plantlets on nearly 50 species
since the concept of photoautotrophic micropropagation was developed more than two decades ago. These studies indicate that
the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous
or porous supporting material with high air porosity instead of gelling agents such as agar. This paper reviews the development
and characteristics of photoautotrophic micropropagation systems and the effects of environmental conditions on the growth
and development of the plantlets. The commercial applications and the perspective of photoautotrophic micropropagation systems
are discussed. 相似文献
9.
10.
SETD3 belongs to a family of SET-domain containing proteins. Recently, SETD3 was found as the first and so-far the only known metazoan histidine methyltransferase that catalyzes actin histidine 73 (His73) methylation, a pervasive modification which was discovered more than 50 years ago. In this review, we summarize some recent advances in SETD3 research, focusing on structural properties, substrate-recognition features, and physiological functions. We particularly highlight potential pathological relevance of SETD3 in human cancers and raise some questions to promote discussion about this novel histidine methyltransferase. 相似文献