全文获取类型
收费全文 | 1107篇 |
免费 | 83篇 |
出版年
2020年 | 7篇 |
2018年 | 13篇 |
2017年 | 10篇 |
2016年 | 22篇 |
2015年 | 31篇 |
2014年 | 26篇 |
2013年 | 43篇 |
2012年 | 53篇 |
2011年 | 57篇 |
2010年 | 42篇 |
2009年 | 26篇 |
2008年 | 53篇 |
2007年 | 40篇 |
2006年 | 59篇 |
2005年 | 44篇 |
2004年 | 38篇 |
2003年 | 40篇 |
2002年 | 38篇 |
2001年 | 33篇 |
2000年 | 41篇 |
1999年 | 39篇 |
1998年 | 11篇 |
1997年 | 13篇 |
1996年 | 15篇 |
1995年 | 8篇 |
1994年 | 22篇 |
1993年 | 9篇 |
1992年 | 24篇 |
1991年 | 14篇 |
1990年 | 23篇 |
1989年 | 17篇 |
1988年 | 20篇 |
1987年 | 18篇 |
1986年 | 11篇 |
1985年 | 16篇 |
1984年 | 12篇 |
1983年 | 13篇 |
1982年 | 7篇 |
1981年 | 14篇 |
1980年 | 8篇 |
1979年 | 14篇 |
1978年 | 13篇 |
1977年 | 10篇 |
1974年 | 6篇 |
1973年 | 11篇 |
1969年 | 5篇 |
1968年 | 6篇 |
1965年 | 5篇 |
1960年 | 6篇 |
1907年 | 5篇 |
排序方式: 共有1190条查询结果,搜索用时 15 毫秒
1.
Usdin K; Chevret P; Catzeflis FM; Verona R; Furano AV 《Molecular biology and evolution》1995,12(1):73-82
The single most difficult problem in phylogenetic analysis is deciding
whether a shared taxonomic character is due to common ancestry or one that
appeared independently due to convergence, parallelism, or reversion to an
ancestral state. Mammalian L1 retrotransposons undergo periodic
amplifications in which multiple copies of the elements are interspersed in
the genome. Because these elements apparently are transmitted only by
inheritance and are retained in the genome, a shared L1 amplification event
can only be an inherited ancestral character. We propose that L1
amplification events can be an excellent tool for analyzing mammalian
evolution and demonstrate here how we addressed several refractory problems
in rodent systematics using L1 DNA as a taxonomic character.
相似文献
2.
Consistent individual differences in animal performance drive individual fitness under variable environmental conditions and provide the framework through which natural selection can operate. Underlying this concept is the assumption that individuals will display consistent levels of performance in fitness-related traits and interest has focused on individual variation and broad sense repeatability in a range of behavioural and physiological traits. Despite playing a central role in maintenance and growth, and with considerable inter-individual variation documented, broad sense repeatability in rates of protein synthesis has not been assessed. In this study we show for the first time that juvenile flounder Platichthys flesus reared under controlled environmental conditions on the same plane of nutrition for 46 days maintain consistent whole-animal absolute rates of protein synthesis (As). By feeding meals containing 15N-labelled protein and using a stochastic end-point model, two non-terminal measures of protein synthesis were made 32 days apart (d14 and d46). As values (mass-corrected to a standard mass of 12 g) showed 2- to 3-fold variation between individuals on d14 and d46 but individuals showed similar As values on both days with a broad sense repeatability estimate of 0.684 indicating significant consistency in physiological performance under controlled experimental conditions. The use of non-terminal methodologies in studies of animal ecophysiology to make repeat measures of physiological performance enables known individuals to be tracked across changing conditions. Adopting this approach, repeat measures of protein synthesis under controlled conditions will allow individual ontogenetic changes in protein metabolism to be assessed to better understand the ageing process and to determine individual physiological adaptive capacity, and associated energetic costs of adaptation, to global environmental change. 相似文献
3.
4.
Electron paramagnetic resonance studies of the tungsten-containing formate dehydrogenase from Clostridium thermoaceticum 总被引:3,自引:0,他引:3
J C Deaton E I Solomon G D Watt P J Wetherbee C N Durfor 《Biochemical and biophysical research communications》1987,149(2):424-430
The redox centers in the tungsten-containing formate dehydrogenase from Clostridium thermoaceticum were examined by potentiometric titration and electron paramagnetic resonance spectroscopy. At low temperature two overlapping iron-sulfur signals which correlated with enzymatic activity were observed with formal potentials near -400 mV vs. SHE. Based on their temperature dependences, one signal is assigned to a reduced Fe2S2 cluster and one to a reduced Fe4S4 cluster. Quantitation of signal intensity suggests two Fe2S2 and two Fe4S4 clusters per formate dehydrogenase molecule. Another signal (g = 2.101, 1.980, 1.950) present in low concentrations at more negative potentials was observable up to 200 degrees K and is not attributed to any iron-sulfur cluster. The possible origin of this signal is analyzed using ligand field theory, and the redox behavior is considered with respect to possible ligation at the active site. 相似文献
5.
Lewis G. Watt Sam Crawshaw Sun-Ju Rhee Alex M. Murphy Toms Canto John P. Carr 《PLoS pathogens》2020,16(12)
The cucumber mosaic virus (CMV) 2b viral suppressor of RNA silencing (VSR) is a potent counter-defense and pathogenicity factor that inhibits antiviral silencing by titration of short double-stranded RNAs. It also disrupts microRNA-mediated regulation of host gene expression by binding ARGONAUTE 1 (AGO1). But in Arabidopsis thaliana complete inhibition of AGO1 is counterproductive to CMV since this triggers another layer of antiviral silencing mediated by AGO2, de-represses strong resistance against aphids (the insect vectors of CMV), and exacerbates symptoms. Using confocal laser scanning microscopy, bimolecular fluorescence complementation, and co-immunoprecipitation assays we found that the CMV 1a protein, a component of the viral replicase complex, regulates the 2b-AGO1 interaction. By binding 2b protein molecules and sequestering them in P-bodies, the 1a protein limits the proportion of 2b protein molecules available to bind AGO1, which ameliorates 2b-induced disease symptoms, and moderates induction of resistance to CMV and to its aphid vector. However, the 1a protein-2b protein interaction does not inhibit the ability of the 2b protein to inhibit silencing of reporter gene expression in agroinfiltration assays. The interaction between the CMV 1a and 2b proteins represents a novel regulatory system in which specific functions of a VSR are selectively modulated by another viral protein. The finding also provides a mechanism that explains how CMV, and possibly other viruses, modulates symptom induction and manipulates host-vector interactions. 相似文献
6.
W B Watt 《The Journal of biological chemistry》1972,247(5):1445-1451
7.
Animal behaviour studies often require the diagnosis and statistical evaluation of patterns among individuals of a group. However, association values derived for all pairs of individuals are mathematically interrelated and, hence, difficult to evaluate. A general method of matrix comparison, called the Mantel test, is described which accounts for such interdependencies. In this test, two square difference matrices are compared to determine whether a statistical association exists between corresponding elements. For example, distances in one of the matrices might represent differences in songs for each pair of a group of birds, while distances in the other matrix could be the geographic distances between each of the same bird pairs; the test would assess whether or not birds close to one another have songs that are more similar than those pairs that are located further apart geographically. Distances in the matrices can be of a variety of sorts (depending on the application), including for instance geographic distances, morphological differences, or behavioural differences. The test has wide applicability in studies of animal behaviour, and we present three examples. First, dialects of splendid sunbirds (Nectarinia coccinigaster) were investigated to determine whether local or regional patterns of geographic variation in song were present, and whether birds with similar dialects were concentrated within the same habitat. Second, dominance hierarchies of white-throated sparrows (Zonotrichia albicollis) and dark-eyed juncos (Junco hyemalis oreganus) were analysed by constructing appropriate hypothesis matrices that were contrasted against a matrix summarizing behavioural interrelationships of flock members. In the third application, involving progressions of yellow baboons (Papio cynocephalus), an approach was developed to assess whether members of a given sex occur adjacent (or at least closer) to one another in progression order more often than expected by chance, and also whether individuals of a given sex tend to be found at or near the end of a progression more frequently than predicted. Other possible applications of the Mantel test are discussed, and a detailed computational example is included. 相似文献
8.
A neonate with aniridia was found to have a one band paracentric inversion of the short arm of chromosome 4. This was initially difficult to interpret on high resolution banding. The inversion was present in three generations of the family. 相似文献
9.
10.