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1.
A new minimal synthetic medium, with low amount of glucose, without aminoacids, vitamins and neutral pH, which induces germ-tubes production in Candida albicans, is reported in this work. The results indicate a perfect agreement between the germ-tube test performed with the standard method in human or animal serum and this test performed in minimal synthetic medium. In this medium the germ-tube test for the presumptive identification of Candida albicans can be performed with the same formality, time and reproducibility as those in human or animal serum. This constitutes an interesting finding because it is easy to prepare, to store and is highly reproducible. 相似文献
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AbstractIn the present work, we have used copper sulphate (CuSO4·5H2O) enriched medium for effective control of visible and latent contamination. Among the different concentrations used, 1.25–2.5?mg/L resulted the most appropriate. In addition, the role of different nitrogen source and concentrations (NH4NO3 and KNO3), as different iron source (FeEDTA and FeEDDHA) has been investigated in the proliferation and rooting phases of European hazelnut (cv. Tonda Gentile Romana). The normal concentration of nitrogen present in Murashige and Skoog medium is too high for hazelnut micropropagation cv. Tonda Gentile Romana. A reduction of total nitrogen, accompanied by a reduction of ammonium forms, resulted in a better quality of the shoots. Similar results have been obtained when the common iron source FeEDTA has been replaced by the same concentration of FeEDDHA. An increase in rooting occurs when the amount of nitrogen was reduced in the rooting medium, particularly when the NH4NO3 was not present. 相似文献
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Different physical and chemical methods were used to detach the chlamydoconidia of Candida albicans from its mycelium. The action of concentrated H2SO4 acid for a 4-min period on cultures lysed both the mycelium and the outer but not the inner wall layer of the chlamydoconidia.The sulfuric acid procedure is recommended as the best method to obtain mycelium free chlamydoconidia because of its simplicity, rapidity and low cost. 相似文献
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Valerio Chiroli Minerva R. Batugo Stefano Biondi Annalisa Bonfanti Stefania Brambilla David C. Gale Lin Li Daniela Miglietta Fabio Nicoli Ganesh A. Prasanna Daniela Ronchetti William F. Vernier Wesley K.M. Chong 《Bioorganic & medicinal chemistry letters》2009,19(10):2785-2788
A novel class of timolol derivatives with nitric oxide (NO)-donating moieties achieved chemical stability yet under physiologically relevant conditions released timolol and NO. Hindered esters A were designed and synthesized, whose ‘triggered’ release relied on enzymatic hydrolysis of the nitrate ester in A to B, that in turn cyclized to liberate timolol. 相似文献
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Sonia Coni Silvia Maria Serrao Zuleyha Nihan Yurtsever Laura Di Magno Rosa Bordone Camilla Bertani Valerio Licursi Zaira Ianniello Paola Infante Marta Moretti Marialaura Petroni Francesca Guerrieri Alessandro Fatica Alberto Macone Enrico De Smaele Lucia Di Marcotullio Giuseppe Giannini Marella Maroder Enzo Agostinelli Gianluca Canettieri 《Cell death & disease》2020,11(12)
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Dott. Valerio Giacomini 《Plant biosystems》2013,147(3):624-648
Riassunto L'A. esamina i componenti fondamentali della flora briologica dell'Africa settentrionale mediterranea, in relazione con i tipi climatici. Distingue un Elemento mediterranco (coi gruppi: stenomediterraneo, euri-mediterraneo, mediterraneo-montano, mediterraneo-atlantico, mediterraneo-steppico, saharo-sindico), atlantico, circumboreale, orofilo-boreale, ipsofilo-boreale, aralo-caspico, cosmopolita ed endemico. 相似文献
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Valerio Mazzoni rea Lucchi rej okl Janez Preern & Meta Virant-Doberlet 《Entomologia Experimentalis et Applicata》2009,133(2):174-185
Scaphoideus titanus Ball (Hemiptera: Cicadellidae: Deltocephalinae) is the vector of the grapevine disease Flavescence dorée. In S. titanus the male–female duet (MFD), based on species-specific vibrational signals, is essential for successful copulation. The female reply within a duet is a single pulse that is coupled with the male pulse with constant latency. It has been shown that a rival male can interrupt an existing duet by emitting disruptive noise signals. We tested whether the reproductive behaviour of S. titanus can be disrupted by the playback of intra-specific and synthesized vibrational signals. Tested males responded to the playback of an MFD with typical rivalry behaviour. Such behaviour includes silent search for a duetting female (satellite behaviour) and/or emission of disruptive signals. These signals were emitted either after exchange of male–female pulses or after two male pulses coupled by latency corresponding to the female response window. The onset of male disruptive signals overlapped with a female pulse. We suggest that the intruder's disruptive signals can mask the female reply and confuse courting males. Playback of disruptive vibrational signals reduced the level of male calling and interrupted an established MFD that consequently resulted in a significantly reduced number of copulations. These results indicate that the vibrational communication channel is open to interference either from abiotic environmental noise or from signals produced by sexual competitors or heterospecifics. The present study also suggests that a detailed understanding of leafhopper behaviour is essential for trying new approaches in the development of more environmentally friendly control practices. 相似文献
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