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Simple method for separating the chlamydoconidia of Candida albicans from its mycelium
Authors:Valerio Vidotto  Massimo Bruatto  Maria Grazia Gallo
Institution:(1) Istituto Malattie Infettive, Università degli Studi di Torino, c. Svizzera 164, 10149 Torino, Italy;(2) Dipartimento di Patologia Animale, Università degli Studi di Torino, Facoltà di Medicina Veterinaria, v. Nizza 52, 10132 Torino, Italy;(3) Present address: Universidad Nadonal San Antonio Abad del Cusco, Cindad Universitaria, Avenida de la Cultura s/n, Apartado Postal 921 Cusco, Perú
Abstract:Different physical and chemical methods were used to detach the chlamydoconidia of Candida albicans from its mycelium. The action of concentrated H2SO4 acid for a 4-min period on cultures lysed both the mycelium and the outer but not the inner wall layer of the chlamydoconidia.The sulfuric acid procedure is recommended as the best method to obtain mycelium free chlamydoconidia because of its simplicity, rapidity and low cost.
Keywords:Candida albicans  separation of chlamydoconidia
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