排序方式: 共有5条查询结果,搜索用时 0 毫秒
1
1.
Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor 下载免费PDF全文
2.
Valeria P. Conforte Florencia Malamud Pablo M. Yaryura Laila Toum Terrones Pablo S. Torres Verónica De Pino Cristian N. Chazarreta Gustavo E. Gudesblat Atilio P. Castagnaro María R. Marano Adrian A. Vojnov 《Molecular Plant Pathology》2019,20(4):589-598
Citrus canker is an important disease of citrus, whose causal agent is the bacterium Xanthomonas citri ssp. citri (Xcc). In previous studies, we found a group of Xcc mutants, generated by the insertion of the Tn5 transposon, which showed impaired ability to attach to an abiotic substrate. One of these mutants carries the Tn5 insertion in hupB, a gene encoding a bacterial histone-like protein, homologue to the β-subunit of the Heat-Unstable (HU) nucleoid protein of Escherichia coli. These types of protein are necessary to maintain the bacterial nucleoid organization and the global regulation of gene expression. Here, we characterized the influence of the mutation in hupB regarding Xcc biofilm formation and virulence. The mutant strain hupB was incapable of swimming in soft agar, whereas its complemented strain partially recovered this phenotype. Electron microscope imaging revealed that impaired motility of hupB was a consequence of the absence of the flagellum. Comparison of the expression of flagellar genes between the wild-type strain and hupB showed that the mutant exhibited decreased expression of fliC (encoding flagellin). The hupB mutant also displayed reduced virulence compared with the wild-type strain when they were used to infect Citrus lemon plants using different infection methods. Our results therefore show that the histone-like protein HupB plays an essential role in the pathogenesis of Xcc through the regulation of biofilm formation and biosynthesis of the flagellum. 相似文献
3.
Brassinosteroid and brassinosteroid-mimic differentially modulate Arabidopsis thaliana fitness under drought 总被引:1,自引:0,他引:1
Pérez-Borroto Lucia Sandra Toum Laila Castagnaro Atilio Pedro González-Olmedo Justo Lorenzo Coll-Manchado Francisco Pardo Esteban Mariano Coll-García Yamilet 《Plant Growth Regulation》2021,95(1):33-47
Plant Growth Regulation - Brassinosteroids (BRs) are widely used to promote plant growth/development and alleviate environmental stresses’ adverse effects. However, its low stability in the... 相似文献
4.
Rivero M Furman N Mencacci N Picca P Toum L Lentz E Bravo-Almonacid F Mentaberry A 《Journal of biotechnology》2012,157(2):334-343
Solanum tuberosum plants were transformed with three genetic constructions expressing the Nicotiana tabacum AP24 osmotine, Phyllomedusa sauvagii dermaseptin and Gallus gallus lysozyme, and with a double-transgene construction expressing the AP24 and lysozyme sequences. Re-transformation of dermaseptin-transformed plants with the AP24/lysozyme construction allowed selection of plants simultaneously expressing the three transgenes. Potato lines expressing individual transgenes or double- and triple-transgene combinations were assayed for resistance to Erwinia carotovora using whole-plant and tuber infection assays. Resistance levels for both infection tests compared consistently for most potato lines and allowed selection of highly resistant phenotypes. Higher resistance levels were found in lines carrying the dermaseptin and lysozyme sequences, indicating that theses proteins are the major contributors to antibacterial activity. Similar results were obtained in tuber infection tests conducted with Streptomyces scabies. Plant lines showing the higher resistance to bacterial infections were challenged with Phytophthora infestans, Rhizoctonia solani and Fusarium solani. Considerable levels of resistance to each of these pathogens were evidenced employing semi-quantitative tests based in detached-leaf inoculation, fungal growth inhibition and in vitro plant inoculation. On the basis of these results, we propose that stacking of these transgenes is a promising approach to achieve resistance to both bacterial and fungal pathogens. 相似文献
5.
Mirguet O Lamotte Y Donche F Toum J Gellibert F Bouillot A Gosmini R Nguyen VL Delannée D Seal J Blandel F Boullay AB Boursier E Martin S Brusq JM Krysa G Riou A Tellier R Costaz A Huet P Dudit Y Trottet L Kirilovsky J Nicodeme E 《Bioorganic & medicinal chemistry letters》2012,22(8):2963-2967
The discovery, synthesis and biological evaluation of a novel series of 7-isoxazoloquinolines is described. Several analogs are shown to increase ApoA1 expression within the nanomolar range in the human hepatic cell line HepG2. 相似文献
1