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Venezuelan red howler monkeys in a semideciduous habitat typically survive injuries and disabilities. Intraspecific physical aggression was the most frequently observed and inferred cause of injury. Thirty-eight percent of 119 howlers of all ages examined during capture had scars or other evidence of “damage.” Overall, the sexes did not differ significantly in total number of injuries or number of individuals classified as “damaged.” The incidence of injury was not independent of age-sex class. The subadult male class had the highest percentage of “damaged” individuals. However, when total injuries per age class were examined for each sex separately, only females showed a pattern that was significantly different than expected based on age (i.e., exposure to injury factors). Subadult females experienced more injuries than expected, whereas adult females had fewer injuries. Troop status (resident troop, natal troop, or extratroop) was not significantly related to the number of injuries in adult and subadult males. Thin finding was not surprising, because adult and subadult males of all status classes are involved in aggression related to breeding competition. Extratroop females had more injuries than expected, and natal females had the fewest injuries. The higher incidence of injuries on subadult females and extratroop females is consistent with aggression-mediated emigration of some females and observed resistance to female immigration by resident females. Overall, 74% of injuries were located on anterior-ventral portions of the body, consistent with the face-to-face fighting observed in howlers. Sociobiological costs of aggression in red howlers are difficult to assess because many howlers, despite frequent and severe injury, subsequently survive and reproduce. We recommend caution in inferring mortality from injuries, especially when social mobility and emigration out of the study area are common. 相似文献
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Theresa K. Tiefenbrunn Philip E. Dawson 《Protein science : a publication of the Protein Society》2009,18(5):970-979
The type 1 repeat domain from thrombospondin has potent antiangiogenic activity and a structurally interesting fold, making it an attractive target for protein engineering. Chemical synthesis is an attractive approach for studying protein domains because it enables the use of unnatural amino acids for site‐specific labeling and detailed structure‐function analysis. Here, we demonstrate the first total chemical synthesis of the thrombospondin type 1 repeat domain by native chemical ligation. In addition to the natural domain, five sites for side chain modification were evaluated and two were found to be compatible with oxidative folding. Several challenges were encountered during peptide synthesis due to the functional complexity of the domain. These challenges were overcome by the use of new solid supports, scavengers, and the testing of multiple ligation sites. We also describe an unusual sequence‐specific protecting group migration observed during cleavage resulting in +90 Da and +194 Da adducts. Synthetic access to this domain enables the synthesis of a number of variants that can be used to further our understanding of the biochemical interaction network of thrombospondin and provide insight into the structure and function of this important antitumorogenic protein domain. 相似文献
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Theresa S.S. Schilhab 《Animal behaviour》2002,63(5):1021-1026
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Abderrahman El Maarouf Damali Moyo-Lee Yaw Theresa Lindhout Damien D. Pearse Warren Wakarchuk Urs Rutishauser 《The Journal of biological chemistry》2012,287(39):32770-32779
In vertebrates, polysialic acid (PSA) is typically added to the neural cell adhesion molecule (NCAM) in the Golgi by PST or STX polysialyltransferase. PSA promotes plasticity, and its enhanced expression by viral delivery of the PST or STX gene has been shown to promote cellular processes that are useful for repair of the injured adult nervous system. Here we demonstrate a new strategy for PSA induction on cells involving addition of a purified polysialyltransferase from Neisseria meningitidis (PSTNm) to the extracellular environment. In the presence of its donor substrate (CMP-Neu5Ac), PSTNm synthesized PSA directly on surfaces of various cell types in culture, including Chinese hamster ovary cells, chicken DF1 fibroblasts, primary rat Schwann cells, and mouse embryonic stem cells. Similarly, injection of PSTNm and donor in vivo was able to produce PSA in different adult brain regions, including the cerebral cortex, striatum, and spinal cord. PSA synthesis by PSTNm requires the presence of the donor CMP-Neu5Ac, and the product could be degraded by the PSA-specific endoneuraminidase-N. Although PSTNm was able to add PSA to NCAM, most of its product was attached to other cell surface proteins. Nevertheless, the PSTNm-induced PSA displayed the ability to attenuate cell adhesion, promote neurite outgrowth, and enhance cell migration as has been reported for endogenous PSA-NCAM. Polysialylation by PSTNm occurred in vivo in less than 2.5 h, persisted in tissues, and then decreased within a few weeks. Together these characteristics suggest that a PSTNm-based approach may provide a valuable alternative to PST gene therapy. 相似文献
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The escape of ornamental plants is a main pathway of invasion into many ecosystems. Non-native plants can alter basal resources and abiotic factors leading to effects that ripple throughout an ecosystem. Invertebrates mediate these effects—responding quickly to abiotic and primary producer changes and, in turn, influencing other species. Invasions are of particular concern in the coastal sage scrub ecosystems of Southern California, where habitat loss and urban encroachment increase invasive species propagule sources and decrease native community resistance. The introduced annual Chrysanthemum coronarium (crown daisy) is a common invader with largely undocumented community-level effects. Our study tested the relationships between the invasive Chrysanthemum and a coastal scrub invertebrate community using a field study at the Tijuana River Estuary. We found similar or lower abundances and diversity of canopy fauna in the presence of Chrysanthemum. Community composition dramatically differed, however, in the presence Chrysanthemum, which was associated with higher abundances of dipterans, wasps and flower beetles, and lower abundances of hemipterans and thysanopterans than native shrubs. Differences in communities were consistent at the species- and order-levels, and were associated with the generally greater plant biomass and shadier conditions afforded by the natives. This study reveals that even a proportionally small amount of Chrysanthemum may shift the invertebrate community through alterations of abiotic properties and plant biomass. We recommend that Chrysanthemum be removed at the first sign of invasion or that spread is prevented since effects on the invertebrate community are dramatic and occur quickly. 相似文献