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Hans Nowack Eckhart Hahn Chella S. David Rupert Timpl Dietrich Götze 《Immunogenetics》1975,2(1):331-335
The genetically controlled immune response to calf skin collagen type I in mice could be demonstrated to be governed by at least two genes. One is linked to theH-2 complex and located within theIA subregion. High-responder alleles areH-2 b ,H-2 f , andH-2 s . The other gene(s) is not linked to theH-2 complex and high-responder allele(s) are found in the genome of B10 mice but not in the genome of DBA mice. There are strong indications that theIr-1A gene controls the response at the T-cell level, whereas it is assumed that the background gene(s) control the immune response at a different level. 相似文献
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Syntheses and stabilities of proteins related to the polyoma small T antigen in Escherichia coli. 总被引:3,自引:1,他引:2
We compared the syntheses and turnovers of two proteins related to the polyoma small T antigen synthesized in Escherichia coli from plasmids containing polyoma genomic segments joined to lac control elements. A protein with an authentic polyoma N terminus was more unstable than a protein with N-terminal amino acids derived from beta-galactosidase. Both were more unstable than most bacterial proteins. 相似文献
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Thomas Josef Vogl Thaddäus T. Wissniowski Nagy N. N. Naguib Renate M. Hammerstingl Martin G. Mack Sabine Münch Matthias Ocker Deike Strobel Eckhart G. Hahn Johannes Hänsler 《Cancer immunology, immunotherapy : CII》2009,58(10):1557-1563
Purpose To asses if laser-induced thermotherapy (LITT) induces a specific cytotoxic T cell response in patients treated with LITT
for colorectal cancer liver metastases.
Methods Eleven patients with liver metastases of colorectal cancer underwent LITT. Blood was sampled before and after LITT. Peripheral
T cell activation was assessed by an interferon gamma (IFNg) secretion assay and flow cytometry. Test antigens were autologous
liver and tumor lysate obtained from each patient by biopsy. T cells were stained for CD3/CD4/CD8 and IFNg to detect activated
T cells. The ratio of IFNg positive to IFNg negative T cells was determined as the stimulation index (SI). To assess cytolytic
activity, T cells were co-incubated with human colorectal cancer cells (CaCo) and cytosolic adenylate kinase release was measured
by a luciferase assay.
Results IFNg secretion assay: before LITT SI was 12.73 (±4.83) for CD3+, 4.36 (±3.32) for CD4+ and 3.64 (±1.77) for CD8+ T cells against
autologous tumor tissue. Four weeks after LITT SI had increased to 92.09 (±12.04) for CD3+ (P < 0.001), 42.92 (±16.68) for CD4+ (P < 0.001) and 47.54 (±15.68) for CD8+ T cells (P < 0.001) against autologous tumor tissue. No increased SI was observed with normal liver tissue at any time point. Cytotoxicity
assay: before LITT activity against the respective cancer cells was low, with RLU = 1,493 (±1,954.68), whereas after LITT
cytolytic activity had increased to RLU = 7,260 [±3,929.76 (P < 0.001)].
Conclusion Patients with liver metastases of colorectal cancer show a tumor-specific cytotoxic T cell stimulation and a significantly
increased cytolytic activity of CD3+, CD4+ and CD8+ T cells after LITT against an allogenic tumor (CaCo cell line). 相似文献
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V. Capozzi P. Russo L. Beneduce S. Weidmann F. Grieco J. Guzzo G. Spano 《Letters in applied microbiology》2010,50(3):327-334
Aims: To isolate indigenous Oenococcus oeni strains suitable as starters for malolactic fermentation (MLF), using a reliable polyphasic approach. Methods and Results: Oenococcus oeni strains were isolated from Nero di Troia wines undergoing spontaneous MLF. Samples were taken at the end of alcoholic fermentation and during MLF. Wine samples were diluted in a sterile physiological solution and plated on MRS and on modified FT80. Identification of O. oeni strains was performed by a polymerase chain reaction (PCR) experiment using strain‐specific primers. Strains were further grouped using a multiplex RAPD‐PCR analysis. Then, six strains were inoculated in two wine‐like media with two different ethanol concentrations (11 and 13% vol/vol) with a view to evaluate their capacity to grow and to perform MLF. In addition, a quantitative PCR (qRT‐PCR) approach was adapted to monitor the physiological state of the strains selected. Conclusion: A positive correlation between the malolactic activity performance and the ability to develop and tolerate stress conditions was observed for two selected O. oeni strains. Significance and Impact of the Study: The results reported are useful for the selection of indigenous MLF starter cultures with desired oenological traits from typical regional wines. It should be the base for the improvement in organoleptic quality of typical red wine. 相似文献
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