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1.
The Saccharomyces cerevisiae MNN4 gene, which is involved inmannosylphosphate transfer from GDP-mannose to N-linked oligosaccharide,has been cloned from a lambda phage containing a yeast chromosomeXI DNA fragment The MNN4 ORF encodes a protein of 1178 aminoacids. The deduced amino acid sequence shows a topology of typeII membrane proteins and has a unique repeated sequence of lysineand glutamic acid at the C-terminus. Disruption and overexpressionof MNN4 led to a decrease and increase, respectively, of themannosylphosphate content in cell wall mannans prepared fromboth mnn4 and wild type strains. A dramatic decrease of mannosylphosphateoccurs in 相似文献
2.
MethodsMice with mutant SOD1 (G93A) transgene, a model for familial ALS, were used in this study. The expression of the major inflammatory cytokines, IL-6, IL-1β and TNF-α, in spinal cords of these SOD1 transgenic (TG) mice were assessed by real time PCR. Mice were then crossed with IL-6(-/-) mice to generate SOD1TG/IL-6(-/-) mice. SOD1 TG/IL-6(-/-) mice (n = 17) were compared with SOD1 TG/IL-6(+/-) mice (n = 18), SOD1 TG/IL-6(+/+) mice (n = 11), WT mice (n = 15), IL-6(+/-) mice (n = 5) and IL-6(-/-) mice (n = 8), with respect to neurological disease severity score, body weight and the survival. We also histologically compared the motor neuron loss in lumber spinal cords and the atrophy of hamstring muscles between these mouse groups.ResultsLevels of IL-6, IL-1β and TNF-α in spinal cords of SOD1 TG mice was increased compared to WT mice. However, SOD1 TG/IL-6(-/-) mice exhibited weight loss, deterioration in motor function and shortened lifespan (167.55 ± 11.52 days), similarly to SOD1 TG /IL-6(+/+) mice (164.31±12.16 days). Motor neuron numbers and IL-1β and TNF-α levels in spinal cords were not significantly different in SOD1 TG /IL-6(-/-) mice and SOD1 TG /IL-6 (+/+) mice.ConclusionThese results provide compelling preclinical evidence indicating that IL-6 does not directly contribute to motor neuron disease caused by SOD1 mutations. 相似文献
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J. Denry Sato Hui-Ting Gao Yoshiaki Kayada Myles C. Cabot Gordon H. Sato Tetsuji Okamoto Clement J. Welsh 《In vitro cellular & developmental biology. Plant》1988,24(12):1223-1228
Summary The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63
mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert [3H]lathosterol to [3H]cholesterol. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due
to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesserone and hydrocortisone could
not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy
characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use
of sterols in defined culture medium for mouse myeloma cells.
This work was supported by National Institute of Health grants CA40294 and CA37589 to G. H. Sato and by a grant from RJR nabisco
Inc.
Editor's Statement These results help identify the defect in myeloma cells leading to cholesterol auxotrophy. The use of these
cells in hybridoma derivation adds practical utility to a detailed appreciation of cholesterol metabolism in these cultures. 相似文献
5.
Ichthyological Research - A taxonomic review of the Sebastes vulpes complex (S. vulpes, S. zonatus and S. ijimae) established the existence of two valid species, Sebastes vulpes Döderlein in... 相似文献
6.
Tetsuji Yamashita Pierre Hakizimana Siva Wu Ahmed Hassan Stefan Jacob Jamshid Temirov Jie Fang Marcia Mellado-Lagarde Richard Gursky Linda Horner Barbara Leibiger Sara Leijon Victoria E. Centonze Per-Olof Berggren Sharon Frase Manfred Auer William E. Brownell Anders Fridberger Jian Zuo 《PLoS genetics》2015,11(9)
Nature’s fastest motors are the cochlear outer hair cells (OHCs). These sensory cells use a membrane protein, Slc26a5 (prestin), to generate mechanical force at high frequencies, which is essential for explaining the exquisite hearing sensitivity of mammalian ears. Previous studies suggest that Slc26a5 continuously diffuses within the membrane, but how can a freely moving motor protein effectively convey forces critical for hearing? To provide direct evidence in OHCs for freely moving Slc26a5 molecules, we created a knockin mouse where Slc26a5 is fused with YFP. These mice and four other strains expressing fluorescently labeled membrane proteins were used to examine their lateral diffusion in the OHC lateral wall. All five proteins showed minimal diffusion, but did move after pharmacological disruption of membrane-associated structures with a cholesterol-depleting agent and salicylate. Thus, our results demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5’s active and structural roles. The structural constraint of membrane proteins may exemplify convergent evolution of cellular motors across species. Our findings also suggest a possible mechanism for disorders of cholesterol metabolism with hearing loss such as Niemann-Pick Type C diseases. 相似文献
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Tetsuji Nakabo Kouji Nakayama Nozomu Muto Masayuki Miyazawa 《Ichthyological Research》2011,58(2):180-183
Oncorhynchus kawamurae (Osteichthyes: Salmonidae) (common name “Kunimasu”), a species endemic to Lake Tazawa, Akita Prefecture, Japan, was believed
to have been extinct since 1940. However, nine specimens were discovered in March and April 2010 in Lake Saiko, Yamanashi
Prefecture, one of the lakes to which eyed eggs of the species were introduced in 1935. These were identified as O. kawamurae because of having 47–62 pyloric caeca, 37–43 gill-rakers, a black-colored body, and spawning at 30–40 m depth in early spring,
which are unique characteristics within Oncorhynchus. Furthermore, the distinctiveness of Kunimasu from sympatric kokanee (O. nerka) was supported by microsatellite DNA data. 相似文献
9.
A new snailfish, Careproctus notosaikaiensis, is described on the basis of 6 specimens collected from the central part of the Sea of Japan, off Saikai, western coast
of the Noto Peninsula, Ishikawa, Japan. The new species is distinguished from other congeners by the following combination
of the characters: vertebrae 57–58, 10–12 abdominal and 46–48 caudal; dorsal-fin rays 52; anal-fin rays 46–47; principal caudal-fin
rays 10; pectoral-fin rays 35–37; pectoral fin with a deep notch; proximal pectoral radials 4 (3 + 1), round; gill slit extending
to pectoral-fin ray 4–7; teeth strongly trilobed; pleural ribs 2 pairs; 2 suprabranchial pores; chin pores paired in the same
pit; pyloric caeca 20–29; dorsal and anal fins with dark margins and stomach black in preserved specimens. 相似文献
10.
A review of East Asian frog flounders, genus Pleuronichthys (family Pleuronectidae), recognized Pleuronichthys japonicus sp. nov. and P. cornutus (Temminck and Schlegel 1846). Pleuronichthys japonicus sp. nov. is characterized by small, dark, rounded spots or marbled markings on the ocular side of the body, rounded cycloid
scales somewhat irregularly arranged, usually 12 abdominal vertebrae, 67–80 (modally 75) dorsal-fin rays, 48–59 (modally 55)
anal-fin rays, and a short branch of the supratemporal lateral line usually present on both sides. Pleuronichthys cornutus is characterized by densely distributed small, dark, irregular spots on the ocular side of the body, elongate cycloid scales
somewhat regularly arranged, usually 13 abdominal vertebrae, 72–88 (modally 77) dorsal-fin rays, 52–65 (modally 58) anal-fin
rays, and a branch of the supratemporal lateral line usually absent on both sides. Whereas P. cornutus is distributed from Miyagi Prefecture (Tohoku District) southward along the Pacific coast of Japan to the Bungo Channel,
from Akita Prefecture (Tohoku District) southward along the Sea of Japan coast through the Tsushima Strait to the East China
Sea, Yellow and Bohai Seas, the Taiwan Strait, and northern Chinese coast of the South China Sea, P. japonicus is distributed from southern Hokkaido southward along the Sea of Japan and Pacific coasts of Japan to the southern East China
Sea. Geographic variations were found in caudal vertebrae and anal-fin ray counts, and caudal-peduncle depth in P. cornutus, and in ocular side body coloration, body depth, and head length in P. japonicus. Pleuronichthys lighti Wu 1929 was regarded as a junior synonym of P. cornutus. 相似文献