Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium |
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Authors: | J Denry Sato Hui-Ting Gao Yoshiaki Kayada Myles C Cabot Gordon H Sato Tetsuji Okamoto Clement J Welsh |
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Institution: | (1) W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, 12946 Lake Placid, New York |
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Abstract: | Summary The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63
mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert 3H]lathosterol to 3H]cholesterol. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due
to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesserone and hydrocortisone could
not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy
characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use
of sterols in defined culture medium for mouse myeloma cells.
This work was supported by National Institute of Health grants CA40294 and CA37589 to G. H. Sato and by a grant from RJR nabisco
Inc.
Editor's Statement These results help identify the defect in myeloma cells leading to cholesterol auxotrophy. The use of these
cells in hybridoma derivation adds practical utility to a detailed appreciation of cholesterol metabolism in these cultures. |
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Keywords: | myeloma cells sterol metabolism 3-ketosteroid reductase serum-free medium |
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