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Rujira Tisarum Thapanee Samphumphung Cattarin Theerawitaya Wittaya Prommee Suriyan Cha-um 《Plant Cell, Tissue and Organ Culture》2018,133(2):215-223
We investigated the effect of carbon dioxide (CO2)-ambient (350 µmol CO2 mol?1) and CO2-enriched (1500 µmol CO2 mol?1) conditions of in vitro photoautotrophic system on two cultivars, ‘RRIM600’ and ‘RRIT413’ of rubber tree (Hevea brasiliensis) in an acclimatization process of 45 days. Survival percentage of in vitro rubber tree plantlets derived from somatic embryos under ambient CO2 was better than those under CO2-enriched conditions, especially in cv. ‘RRIT413’. Subsequently, the survival rate of ex vitro transplanted plantlets was similar to the in vitro plantlets and abnormal morphological characters such as light-green leaves (SPAD), small leaves in cv. ‘RRIT413’ acclimatized under CO2-enriched conditions were demonstrated 30 days after the plantlets were transferred into the soil. Maximum quantum yield of PSII, photon yield of PSII, stomatal conductance and transpiration rate in cv. ‘RRIT413’ acclimatized under CO2-enriched conditions were sharply declined by 39.0, 50.6, 47.1 and 45.8%, respectively as compared to those acclimatized under ambient CO2 conditions. In contrast, the in vitro acclimatized plantlets of cv. ‘RRIM600’ were un-responsive under both ambient- and enriched-CO2 conditions. In conclusion, genotypic dependent in response to CO2 enriched conditions in in-vitro acclimatization of rubber tree plantlets was evidently demonstrated as a key result to regulate plant growth and development in ex vitro environments. Interestingly, soluble sugar contents (sucrose, glucose and fructose) were increased after transplanting the plantlets of cv. ‘RRIM600’ acclimatized under CO2-enriched condition into the soil and thus, can be considered as an adaptive indicator of ex vitro adaptation. 相似文献
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Suriyan Tunkijjanukij Hanne Giæver Christopher C.Q Chin Jan A Olafsen 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1998,119(4):705-713
Sialic acids have been implicated in a variety of complex biological regulatory and signalling events and their functional importance is reflected by their presence in a wide variety of phyla. Potentially they may inhibit intermolecular and intercellular interactions. Lectins that exhibit specificity for sialic acid or sialoglycoconjugates are ubiquitous in the body fluids of invertebrates and this has supported the assumption that these lectins are involved in defense against microbes that express sialic acids on their surfaces. This biological function has also been inferred from the absence of sialic acids in lower invertebrates. However, most invertebrate lectins are heterogeneous and may also bind other ligands. The biological significance of the different carbohydrate specificities are not yet known. We have demonstrated the presence of sialic acids in hemolymph from two marine bivalves, the Pacific oyster Crassostrea gigas (≈15 μg ml−1) and the horse mussel Modiolus modiolus (48–100 μg ml−1) by several different assays. The sialic acid was mostly in free form. Affinity purified lectins from the horse mussel also contained bound sialic acids (2–5 μmol g−1). Oyster hemolymph stimulated the in vitro phagocytosis of bacteria by oyster hemocytes. The stimulation by hemolymph is facilitated by a dialyzable component, that apparently is active irrespective of the binding to sialic acid (BSM). Addition of sialic acid had no significant effect on the in vitro phagocytosis of bacteria by oyster hemocytes. 相似文献
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Suriyan Cha-um Thapanee Samphumphuang Chalermpol Kirdmanee 《In vitro cellular & developmental biology. Plant》2012,48(2):259-264
Nodal explants of rice cultivar Pathumthani 1 (PT1; short-day photoperiod insensitive) were collected, surface-disinfected,
and cultured on modified MS medium under in vitro conditions for 90 d. A total of 60% nodal explants generated flowering plantlets (with one inflorescence per cluster). The
net photosynthetic rate was greater, and soluble sugars (including glucose, fructose, and sucrose) accumulated to higher levels
in the leaves of flowering as compared to non-flowering plants. In contrast, chlorophyll a, chlorophyll b, total chlorophyll,
and total carotenoid content were enriched to a greater degree in the leaves of non-flowering as compared to flowering plants.
Also, growth performance parameters, including plant height, number of leaves per plant, leaf area, fresh weight, and dry
weight of plantlets derived from seedlings were superior to those of plantlets derived from nodal explants. In addition, the
protocol proved to successfully induce flowering in KDML 105, a short-day photoperiod-sensitive rice cultivar. 相似文献
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Suravoot Yooyongwech Nuttawuth Phaukinsang Suriyan Cha-um Kanyaratt Supaibulwatana 《Plant Growth Regulation》2013,69(3):285-293
Water deficit limits plant growth and yield. Arbuscular mycorrhizal (AM) symbiosis is viewed as one of the several methods to improve growth under water deficit. The present study investigated the growth performance in relation to water deficit in two cultivars (“H2” and “660”) of AM treated macadamia (Macadamia tetraphylla L.) plants. AM treatment significantly improved the growth in macadamia plants that have been subjected to water deficit (7 % soil water content) for 14 days. Leaf water content (LWC) and maximum quantum yield of PSII (Fv/Fm) in AM-associated plants were maintained better than those in the control (well-watered) plants. A positive correlation was observed between LWC and Fv/Fm in “H2” cultivar. AM treatment enhanced proline and soluble sugar content in “H2” cultivar under water deficit stress. In contrast, only soluble sugars were accumulated in the AM-associated plants of “660” cultivar under water deficit stress. The study concludes that soluble sugars and proline are involved as key signals of osmoregulation defense response, improve water relation in plant tissues, and thereby resulting in improved growth in AM-associated macadamia plants. 相似文献
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Cattarin?Theerawitaya Nana?Yamada-Kato Harminder?Pal?Singh Suriyan?Cha-um Teruhiro?TakabeEmail author 《Protoplasma》2018,255(5):1423-1432
The plant specific DREPP proteins have been shown to bind Ca2+ and regulate the N-myristoylation signaling and microtubule polymerization in Arabidopsis thaliana. The information about DREPP proteins in other plants is, however, scarce. In the present study, we isolated the DREPP gene from a halophytic grass, Sporobolus virginicus, and tested whether the gene was involved in alkaline salt stress responses. The SvDREPP1 was cloned from S. virginicus by RACE methods. The isolated gene showed high homology to DREPP homologs from C4 grasses, Setaria italica, and Panicum hallii as well as rice (OsDREPP1). The encoded protein contained 202 amino acid residues. It was expressed in E. coli, and its biochemical properties were studied. It was observed that SvDREPP1 was not only Ca2+-binding protein, but also bind to calmodulin and microtubules. The SvDREPP1 mRNA expression in plants grown under alkaline salt stress was upregulated by 3.5 times over the control in leaf tissues after 48-h treatment, whereas it was increased for 6.0 times in the root tissues at 36 h. The data suggests the importance of SvDREPP1 in regulating alkali salt stress responses in the leaf tissues. 相似文献
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Ponnusamy S Alderson NL Hama H Bielawski J Jiang JC Bhandari R Snyder SH Jazwinski SM Ogretmen B 《The Journal of biological chemistry》2008,283(41):27514-27524
In this study, we investigated the roles of very long-chain fatty acid (VLCFA) synthesis by fatty acid elongase 3 (ELO3) in the regulation of telomere length and life span in the yeast Saccharomyces cerevisiae. Loss of VLCFA synthesis via deletion of ELO3 reduced telomere length, and reconstitution of the expression of wild type ELO3, and not by its mutant with decreased catalytic activity, rescued telomere attrition. Further experiments revealed that alterations of phytoceramide seem to be dispensable for telomere shortening in response to loss of ELO3. Interestingly, telomere shortening in elo3Delta cells was almost completely prevented by deletion of IPK2 or KCS1, which are involved in the generation of inositol phosphates (IP4, IP5, and inositol pyrophosphates). Deletion of IPK1, which generates IP6, however, did not affect regulation of telomere length. Further data also suggested that elo3Delta cells exhibit accelerated chronologic aging, and reduced replicative life span compared with wild type cells, and deletion of KCS1 helped recover these biological defects. Importantly, to determine downstream mechanisms, epistasis experiments were performed, and data indicated that ELO3 and YKU70/80 share a common pathway for the regulation of telomere length. More specifically, chromatin immunoprecipitation assays revealed that the telomere binding and protective function of YKu80p in vivo was reduced in elo3Delta cells, whereas its non-homologues end-joining function was not altered. Deletion of KCS1 in elo3Delta cells recovered the telomere binding and protective function of Ku, consistent with the role of KCS1 mutation in the rescue of telomere length attrition. Thus, these findings provide initial evidence of a possible link between Elo3-dependent VLCFA synthesis, and IP metabolism by KCS1 and IPK2 in the regulation of telomeres, which play important physiological roles in the control of senescence and aging, via a mechanism involving alterations of the telomere-binding/protection function of Ku. 相似文献
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Suriyan Cha-um Chaturong Chanseetis Watchara Chintakovid Aussanee Pichakum Kanyaratt Supaibulwatana 《Plant Cell, Tissue and Organ Culture》2011,106(3):435-444
In this study, a rooting protocol was developed for macadamia plantlets with healthy roots and enhanced growth performance,
along with enhanced photosynthetic capability. In vitro-grown shoots rooted in vented vessels containing vermiculite as the
supporting material exhibited 100% frequency of root induction, whereas when shoots were grown in non-vented vessels containing
a solidified Murashige and Skoog (MS) medium, the frequency of root induction was less than 30%. The formation of root with
callus, hyperhydricity, and leaf necrosis was observed in this photomixotrophic closed system. The modification of the vented
photoautotrophic system with different concentrations of CO2 and sucrose were investigated using vermiculite as the supporter. The number of roots, root length, root surface area, fresh
weight, and dry weight were significantly higher in plantlets grown in CO2-enriched (1,000 μmol CO2 mol−1) photoautotrophic conditions. The water content in both root and shoot tissues of plantlets cultured under photoautotrophic
conditions was maximized. In addition, shoot and leaf performances were enhanced in plantlets cultured under CO2-enriched photoautotrophic conditions. The supplementation of sucrose (29–88 mM) to culture media in both ambient and elevated
CO2 conditions affected a reduction in the shoot and root performance of in vitro plantlets. Chlorophyll a, chlorophyll b, and
total carotenoids in the leaf tissues of plantlets acclimatized in CO2-enriched photoautotrophic conditions were enriched, leading to increasing photosynthetic abilities, including chlorophyll
fluorescence and net photosynthetic rate. From this investigation, a root induction protocol was established and the production
of healthy macadamia plantlets was successfully implemented using CO2-enriched photoautotrophic conditions. 相似文献
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Suriyan Cha-um Bootsaya Srianan Aussanee Pichakum Chalermpol Kirdmanee 《In vitro cellular & developmental biology. Plant》2009,45(2):171-179
Anther culture is a biotechnology technique that can be used for the production of pure lines. The aims of this investigation
were to induce embryogenic callus from major and minor culms of Thai aromatic rice cultivars and to subsequently regenerate
double-haploid green plantlets by the application of exogenous polyamines. Embryogenic callus derived from anther culture
was successfully induced in varieties KDML105, Homjan (HJ), and Pathumthani 1 (PT1). Production of embryogenic callus from
anthers collected from the major culms was greater than those collected from the minor culms, especially in cultivar HJ. Plantlet
regeneration in the three rice cultivars was observed from embryogenic callus and was highest, at 12.1%, from variety HJ treated
with 0.5 mM spermidine. Plantlet regeneration from anther-derived embryogenic callus was dependent on the plant genotype,
the types of exogenous polyamines, and the interactions of these factors. The percentage of haploid plantlets regenerated
in PT1, KDML105, and HJ were 68.1%, 70.7%, and 78.5%, respectively. Only haploid plantlets were treated with colchicine for
double-haploid production. This investigation has increased the knowledge of both embryogenic callus induction and plantlet
regeneration in aromatic rice and has lead to the development of a pure, double-haploid line for the use in rice breeding
programs in Thailand. 相似文献