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Callus cultures from salt tolerant (CSR-10) and susceptible (Swarnadhan) varieties of Oryza sativa L. were established in Murashige and Skoog’s (MS) medium containing lethal concentrations (50 mM) of rubidium chloride (RbCl)
as a selective agent. While 95–100% cells were viable in callus cultures grown without RbCl, viability was 75% in 50 mM RbCl
selected cultures. Growth of RbCl selected calli in presence of salt was comparable to that of callus grown without it. Cells
tolerant to RbCl showed more vacuoles and accumulated more K+ in comparison with their corresponding controls. Suspension cultures were established and uptake of 86Rb+ was measured at 10 and 20 min intervals, which revealed a linear relationship between the absorption of K+ and time. Callus cultures (560-day-old) tolerant to 50 mM RbCl regenerated shoots with 35–40% frequencies in both the varieties,
but the same age-old callus grown in the medium devoid of RbCl did not show any organogenesis. Callus cultures that are tolerant
to 50 mM RbCl when exposed to 25 mM LiCl, 50 mM NaCl, 50 mM KCl and 25 mM CsCl also exhibited cross tolerance in both the
varieties. This is the first time that a callus line of rice resistant to RbCl was raised and shown to accumulate a major
cation K+ and also an increased influx of it. 相似文献
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Neelima Arora Amit Kumar Banerjee Srilaxmi Mutyala Upadhyayula Suryanarayana Murty 《Bioinformation》2009,3(10):446-453
Xylanase is an industrially important enzyme having wide range of applications especially in paper industry. It is crucial to gain
an understanding about the structure and functional aspects of various xylanases produced from diverse sources. In this study, a
bioinformatics and molecular modeling approach was adopted to explore properties and structure of xylanases. Physico-chemical
properties were predicted and prediction of motifs, disulfide bridges and secondary structure was performed for functional
characterization. Apart from these analyses, three dimensional structures were constructed and stereo-chemical quality was
evaluated by different structure validation tools. Comparative catalytic site analysis and assessment was performed to extract
information about the important residues. Asn72 was found to be the common residue in the active sites of the proteins
and P35809. Q12603相似文献
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Shravankumar Kankala Ranjith Kumar Kankala Prasad Gundepaka Niranjan Thota Srinivas Nerella Mohan Rao Gangula Hanmanthu Guguloth Mukkanti Kagga Ravinder Vadde Chandra Sekhar Vasam 《Bioorganic & medicinal chemistry letters》2013,23(5):1306-1309
Regioselective synthesis of isoxazole–mercaptobenzimidazole hybrids and their efficiency in in vivo analgesic and anti-inflammatory activity was described. A comparison of structure–activity relationship for there compounds was also emphasized. 相似文献
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Karolina A. Aberg Lin Y. Xie Srilaxmi Nerella William E. Copeland E. Jane Costello Edwin J.C.G. van den Oord 《Epigenetics》2013,8(5):542-547
The potential importance of DNA methylation in the etiology of complex diseases has led to interest in the development of methylome-wide association studies (MWAS) aimed at interrogating all methylation sites in the human genome. When using blood as biomaterial for a MWAS the DNA is typically extracted directly from fresh or frozen whole blood that was collected via venous puncture. However, DNA extracted from dry blood spots may also be an alternative starting material. In the present study, we apply a methyl-CpG binding domain (MBD) protein enrichment-based technique in combination with next generation sequencing (MBD-seq) to assess the methylation status of the ~27 million CpGs in the human autosomal reference genome. We investigate eight methylomes using DNA from blood spots. This data are compared with 1,500 methylomes previously assayed with the same MBD-seq approach using DNA from whole blood. When investigating the sequence quality and the enrichment profile across biological features, we find that DNA extracted from blood spots gives comparable results with DNA extracted from whole blood. Only if the amount of starting material is ≤ 0.5µg DNA we observe a slight decrease in the assay performance. In conclusion, we show that high quality methylome-wide investigations using MBD-seq can be conducted in DNA extracted from archived dry blood spots without sacrificing quality and without bias in enrichment profile as long as the amount of starting material is sufficient. In general, the amount of DNA extracted from a single blood spot is sufficient for methylome-wide investigations with the MBD-seq approach. 相似文献
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